Shotgun Lipidomic Analysis of Leishmania Cells

Kai Zhang, Fong Fu Hsu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

To understand the interplay of lipids between Leishmania promastigotes, amastigotes, and vertebrate host cells, a robust method for cultivating Leishmania parasites, lipid extraction, and shotgun lipidomic analysis with loop injection is described. This book chapter provides the step-by-step workflow to guide readers from sample preparation to the global lipid analysis by multiple stage mass spectrometry with high resolution and tandem quadrupole mass spectrometric approaches toward studying the metabolomic roles that lipids may play in Leishmania parasite infections.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages215-225
Number of pages11
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2306
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Glycerophospholipids
  • High-resolution mass spectrometry
  • Leishmania lipids
  • Sphingolipids

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