Abstract
Glycosylation is one of the most common and complex post-translational modifications of proteins. However, there are other carbohydrates such as free oligosaccharides and glycosphingolipids-glycans that are associated with important biological and clinical roles. To analyze these molecules using liquid chromatography coupled with mass spectrometry (LC-MS), the permethylation approach was utilized. Although permethylation is a commonly utilized glycan derivatization technique, separation of permethylated glycans released from glycosphingolipid (GSL) by LC-MS has never been previously demonstrated. Here, a nanoflow porous graphitized carbon (PGC) column coupled with a high-resolution mass spectrometer was used to achieve isomeric separation of these permethylated glycans. We demonstrate the separation of free reducing end and reduced end O-glycans, free oligosaccharides derived from human milk, and GSL glycans derived from the MDA-MB-231BR cancer cell line using PGC-LC-MS.
Original language | English |
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Article number | 433 |
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Metabolites |
Volume | 10 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2020 |
Keywords
- Free oligosaccharides
- Glycomics
- Glycosphingolipids
- LC-MS
- O-glycans
- PGC