Separation of permethylated o-glycans, free oligosaccharides, and glycosphingolipid-glycans using porous graphitized carbon (Pgc) column

Byeong Gwan Cho, Wenjing Peng, Yehia Mechref

Research output: Contribution to journalArticlepeer-review

Abstract

Glycosylation is one of the most common and complex post-translational modifications of proteins. However, there are other carbohydrates such as free oligosaccharides and glycosphingolipids-glycans that are associated with important biological and clinical roles. To analyze these molecules using liquid chromatography coupled with mass spectrometry (LC-MS), the permethylation approach was utilized. Although permethylation is a commonly utilized glycan derivatization technique, separation of permethylated glycans released from glycosphingolipid (GSL) by LC-MS has never been previously demonstrated. Here, a nanoflow porous graphitized carbon (PGC) column coupled with a high-resolution mass spectrometer was used to achieve isomeric separation of these permethylated glycans. We demonstrate the separation of free reducing end and reduced end O-glycans, free oligosaccharides derived from human milk, and GSL glycans derived from the MDA-MB-231BR cancer cell line using PGC-LC-MS.

Original languageEnglish
Article number433
Pages (from-to)1-12
Number of pages12
JournalMetabolites
Volume10
Issue number11
DOIs
StatePublished - Nov 2020

Keywords

  • Free oligosaccharides
  • Glycomics
  • Glycosphingolipids
  • LC-MS
  • O-glycans
  • PGC

Fingerprint Dive into the research topics of 'Separation of permethylated o-glycans, free oligosaccharides, and glycosphingolipid-glycans using porous graphitized carbon (Pgc) column'. Together they form a unique fingerprint.

Cite this