TY - JOUR
T1 - Salmonella in beef and produce from honduras
AU - Maradiaga, Martha
AU - Miller, Mark F.
AU - Thompson, Leslie
AU - Pond, Ansen
AU - Gragg, Sara E.
AU - Echeverry, Alejandro
AU - Garcia, Lyda G.
AU - Loneragan, Guy H.
AU - Brashears, Mindy M.
N1 - Publisher Copyright:
© International Association for Food Protection.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Salmonella continues to cause a considerable number of foodborne illnesses worldwide. The sources of outbreaks include contaminated meat and produce. The purpose of this study was to establish an initial investigation of the burden of Salmonella in produce and beef from Honduras by sampling retail markets and abattoirs. Retail produce samples (cantaloupes, cilantro, cucumbers, leafy greens, peppers, and tomatoes; n ∼ 573) were purchased in three major cities of Honduras, and retail wholemuscle beef (n ∼ 555) samples were also purchased in four major cities. Additionally, both hide and beef carcass (n ∼ 141) samples were collected from two Honduran abattoirs. Whole-muscle beef samples were obtained using a sponge hydrated with buffered peptone water, and 10 ml of the buffered peptone water rinsate of each produce sample was collected with a dry sponge and placed in a bag to be transported back to the United States. Salmonella was detected using a commercially available, closeplatform PCR system, and positive samples were subjected to culture on selective media to obtain isolates. Overall, the prevalence of Salmonella-positive samples, based on PCR detection in Honduras (n ∼ 555) retail beef was 10.1% (95% confidence interval ∼ 7.8, 12.9), whereas 7.8% (n ∼ 141) of beef carcass and hides samples were positive in both beef plants. The overall Salmonella prevalence for all produce samples (n ∼ 573) collected was 2.1% (95% confidence interval ∼ 1.2, 3.6). The most common serotypes identified in Honduras were Salmonella Typhimurium followed by Derby. These results provide an indication of Salmonella contamination of beef and produce in Honduras. Developing a Salmonella baseline for Latin America through an initial investigation like the one presented here contributes to a broader global understanding of the potential exposure through food, thus providing insight into the needs for control strategies.
AB - Salmonella continues to cause a considerable number of foodborne illnesses worldwide. The sources of outbreaks include contaminated meat and produce. The purpose of this study was to establish an initial investigation of the burden of Salmonella in produce and beef from Honduras by sampling retail markets and abattoirs. Retail produce samples (cantaloupes, cilantro, cucumbers, leafy greens, peppers, and tomatoes; n ∼ 573) were purchased in three major cities of Honduras, and retail wholemuscle beef (n ∼ 555) samples were also purchased in four major cities. Additionally, both hide and beef carcass (n ∼ 141) samples were collected from two Honduran abattoirs. Whole-muscle beef samples were obtained using a sponge hydrated with buffered peptone water, and 10 ml of the buffered peptone water rinsate of each produce sample was collected with a dry sponge and placed in a bag to be transported back to the United States. Salmonella was detected using a commercially available, closeplatform PCR system, and positive samples were subjected to culture on selective media to obtain isolates. Overall, the prevalence of Salmonella-positive samples, based on PCR detection in Honduras (n ∼ 555) retail beef was 10.1% (95% confidence interval ∼ 7.8, 12.9), whereas 7.8% (n ∼ 141) of beef carcass and hides samples were positive in both beef plants. The overall Salmonella prevalence for all produce samples (n ∼ 573) collected was 2.1% (95% confidence interval ∼ 1.2, 3.6). The most common serotypes identified in Honduras were Salmonella Typhimurium followed by Derby. These results provide an indication of Salmonella contamination of beef and produce in Honduras. Developing a Salmonella baseline for Latin America through an initial investigation like the one presented here contributes to a broader global understanding of the potential exposure through food, thus providing insight into the needs for control strategies.
UR - http://www.scopus.com/inward/record.url?scp=84923813700&partnerID=8YFLogxK
U2 - 10.4315/0362-028X.JFP-14-450
DO - 10.4315/0362-028X.JFP-14-450
M3 - Article
C2 - 25719872
AN - SCOPUS:84923813700
SN - 0362-028X
VL - 78
SP - 498
EP - 502
JO - Journal of food protection
JF - Journal of food protection
IS - 3
ER -