RNA extraction.

Huazhong Shi, Ray Bressan

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

RNA extraction is a routine technique in a molecular biology lab. High quality of RNA extracted from plants is a prerequisite to succeeding in subsequent experiments such as reverse transcription-polymerase chain reaction, Northern hybridization, cDNA library construction, and microarray analysis. Two methods for RNA extraction in Arabidopsis, small-scale and large-scale, are described here. RNA isolated by using the small-scale method can be applied in most downstream applications. The large-scale RNA preparation is recommended to extract RNA for Northern hybridization only.

Original languageEnglish
Pages (from-to)345-348
Number of pages4
JournalMethods in molecular biology (Clifton, N.J.)
Volume323
DOIs
StatePublished - 2006

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