RNA extraction.

Huazhong Shi; Ray Bressan

doi:10.1385/1-59745-003-0:345

RNA extraction.

Huazhong Shi, Ray Bressan

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

RNA extraction is a routine technique in a molecular biology lab. High quality of RNA extracted from plants is a prerequisite to succeeding in subsequent experiments such as reverse transcription-polymerase chain reaction, Northern hybridization, cDNA library construction, and microarray analysis. Two methods for RNA extraction in Arabidopsis, small-scale and large-scale, are described here. RNA isolated by using the small-scale method can be applied in most downstream applications. The large-scale RNA preparation is recommended to extract RNA for Northern hybridization only.

Original language	English
Pages (from-to)	345-348
Number of pages	4
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	323
DOIs	https://doi.org/10.1385/1-59745-003-0:345
State	Published - 2006

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AB - RNA extraction is a routine technique in a molecular biology lab. High quality of RNA extracted from plants is a prerequisite to succeeding in subsequent experiments such as reverse transcription-polymerase chain reaction, Northern hybridization, cDNA library construction, and microarray analysis. Two methods for RNA extraction in Arabidopsis, small-scale and large-scale, are described here. RNA isolated by using the small-scale method can be applied in most downstream applications. The large-scale RNA preparation is recommended to extract RNA for Northern hybridization only.

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RNA extraction.

Abstract

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