TY - JOUR
T1 - Rhizobacterial volatile emissions regulate auxin homeostasis and cell expansion in Arabidopsis
AU - Zhang, Huiming
AU - Kim, Mi Seong
AU - Krishnamachari, Venkat
AU - Payton, Paxton
AU - Sun, Yan
AU - Grimson, Mark
AU - Farag, Mohamed A.
AU - Ryu, Choong Min
AU - Allen, Randy
AU - Melo, Itamar S.
AU - Paré, Paul W.
N1 - Funding Information:
Acknowledgments We especially want to thank Drs. Rangasamy Elumalai for technical expertise with microarray analyses; Mary Catherine Hastert for technical expertise with microscopic analyses; and Tom Guilfoyle for the DR5::GUS line. This research was funded in part by the Welch Foundation (Grant D1478), The Frasch Foundation for Chemical Research, Biogreen 21 and Technology Development Programs for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.
PY - 2007/9
Y1 - 2007/9
N2 - Certain plant growth-promoting rhizobacteria (PGPR), in the absence of physical contact with a plant stimulate growth via volatile organic compound (VOC) emissions, through largely unknown mechanisms. To probe how PGPR VOCs trigger growth in plants, RNA transcript levels of Arabidopsis seedlings exposed to Bacillus subtilus (strain GB03) were examined using oligonucleotide microarrays. In screening over 26,000 protein-coded transcripts, a group of approximately 600 differentially expressed genes related to cell wall modifications, primary and secondary metabolism, stress responses, hormone regulation and other expressed proteins were identified. Transcriptional and histochemical data indicate that VOCs from the PGPR strain GB03 trigger growth promotion in Arabidopsis by regulating auxin homeostasis. Specifically, gene expression for auxin synthesis was up regulated in aerial regions of GB03-exposed plants; auxin accumulation decreased in leaves and increased in roots with GB03 exposure as revealed in a transgenic DR5::GUS Arabidopsis line, suggesting activation of basipetal auxin transport. Application of the auxin transport inhibitor 1-naphthylphthalamic acid (NPA) restricted auxin accumulation to sites of synthesis thereby preventing GB03-mediated decreases in shoot auxin levels as well as thwarting GB03-mediated growth promotion. In addition, microarray data revealed coordinated regulation of cell wall loosening enzymes that implicated cell expansion with GB03 exposure, which was confirmed by comparative cytological measurements. The discovery that bacterial VOCs, devoid of auxin or other known plant hormones regulate auxin homeostasis and cell expansion provides a new paradigm as to how rhizobacteria promote plant growth.
AB - Certain plant growth-promoting rhizobacteria (PGPR), in the absence of physical contact with a plant stimulate growth via volatile organic compound (VOC) emissions, through largely unknown mechanisms. To probe how PGPR VOCs trigger growth in plants, RNA transcript levels of Arabidopsis seedlings exposed to Bacillus subtilus (strain GB03) were examined using oligonucleotide microarrays. In screening over 26,000 protein-coded transcripts, a group of approximately 600 differentially expressed genes related to cell wall modifications, primary and secondary metabolism, stress responses, hormone regulation and other expressed proteins were identified. Transcriptional and histochemical data indicate that VOCs from the PGPR strain GB03 trigger growth promotion in Arabidopsis by regulating auxin homeostasis. Specifically, gene expression for auxin synthesis was up regulated in aerial regions of GB03-exposed plants; auxin accumulation decreased in leaves and increased in roots with GB03 exposure as revealed in a transgenic DR5::GUS Arabidopsis line, suggesting activation of basipetal auxin transport. Application of the auxin transport inhibitor 1-naphthylphthalamic acid (NPA) restricted auxin accumulation to sites of synthesis thereby preventing GB03-mediated decreases in shoot auxin levels as well as thwarting GB03-mediated growth promotion. In addition, microarray data revealed coordinated regulation of cell wall loosening enzymes that implicated cell expansion with GB03 exposure, which was confirmed by comparative cytological measurements. The discovery that bacterial VOCs, devoid of auxin or other known plant hormones regulate auxin homeostasis and cell expansion provides a new paradigm as to how rhizobacteria promote plant growth.
KW - Auxin transport
KW - Bacillus subtilus GB03
KW - Cell expansion
KW - Plant growth promotion
KW - Rhizobacterial signaling
KW - Transcriptional profiling
UR - http://www.scopus.com/inward/record.url?scp=34548030184&partnerID=8YFLogxK
U2 - 10.1007/s00425-007-0530-2
DO - 10.1007/s00425-007-0530-2
M3 - Article
C2 - 17497164
AN - SCOPUS:34548030184
VL - 226
SP - 839
EP - 851
JO - Planta
JF - Planta
SN - 0032-0935
IS - 4
ER -