Responses of Escherichia coli and Listeria monocytogenes to ozone on non-host tomato: Efficacy of intervention and evidence of induced acclimation

Xiaomei Shu; Manavi Singh; Naga Bhushana R Karampudi; David Bridges; Ai Kitazumi; Vivian C.H. Wu; Benildo de los Reyes

doi:10.1371/journal.pone.0256324

Responses of Escherichia coli and Listeria monocytogenes to ozone on non-host tomato: Efficacy of intervention and evidence of induced acclimation

Xiaomei Shu, Manavi Singh, Naga Bhushana R Karampudi, David Bridges, Ai Kitazumi, Vivian C.H. Wu, Benildo de los Reyes

Plant & Soil Science

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Abstract

Because of the continuous rise of foodborne illnesses caused by the consumption of raw fruits and vegetables, effective post-harvest anti-microbial strategies are needed. This study evaluated the dose × time effects on antimicrobial action of ozone (O3) gas on the the Gram-negative Escherichia coli O157:H7 and Gram-positive Listeria monocytogenes, which are common contaminants in fresh produce. The study on a non-host tomato environment correlated the dose × time aspects of xenobiosis by examining the correlation between bacterial survival in terms of log-reduction and responses at the level of gene expression. In E. coli O157:H7, low (1 µg O3/g of fruit) and moderate (2 µg O3/g of fruit) doses caused insignificant reduction in survival, while a high dose (3 µg/g of fruit) caused significant reduction in survival in a time-dependent manner. In L. monocyt

Original language	English
Pages (from-to)	e0256324
Journal	PLoS ONE
DOIs	https://doi.org/10.1371/journal.pone.0256324
State	Published - Oct 2021

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10.1371/journal.pone.0256324

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title = "Responses of Escherichia coli and Listeria monocytogenes to ozone on non-host tomato: Efficacy of intervention and evidence of induced acclimation",

abstract = "Because of the continuous rise of foodborne illnesses caused by the consumption of raw fruits and vegetables, effective post-harvest anti-microbial strategies are needed. This study evaluated the dose × time effects on antimicrobial action of ozone (O3) gas on the the Gram-negative Escherichia coli O157:H7 and Gram-positive Listeria monocytogenes, which are common contaminants in fresh produce. The study on a non-host tomato environment correlated the dose × time aspects of xenobiosis by examining the correlation between bacterial survival in terms of log-reduction and responses at the level of gene expression. In E. coli O157:H7, low (1 µg O3/g of fruit) and moderate (2 µg O3/g of fruit) doses caused insignificant reduction in survival, while a high dose (3 µg/g of fruit) caused significant reduction in survival in a time-dependent manner. In L. monocyt",

author = "Xiaomei Shu and Manavi Singh and Karampudi, {Naga Bhushana R} and David Bridges and Ai Kitazumi and Wu, {Vivian C.H.} and {de los Reyes}, Benildo",

year = "2021",

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doi = "10.1371/journal.pone.0256324",

language = "English",

pages = "e0256324",

journal = "PLoS ONE",

publisher = "Public Library of Science",

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Responses of Escherichia coli and Listeria monocytogenes to ozone on non-host tomato: Efficacy of intervention and evidence of induced acclimation. / Shu, Xiaomei; Singh, Manavi; Karampudi, Naga Bhushana R; Bridges, David; Kitazumi, Ai; Wu, Vivian C.H.; de los Reyes, Benildo.

In: PLoS ONE, 10.2021, p. e0256324.

Research output: Contribution to journal › Article › peer-review

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AU - Shu, Xiaomei

AU - Singh, Manavi

AU - Karampudi, Naga Bhushana R

AU - Bridges, David

AU - Kitazumi, Ai

AU - Wu, Vivian C.H.

AU - de los Reyes, Benildo

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AB - Because of the continuous rise of foodborne illnesses caused by the consumption of raw fruits and vegetables, effective post-harvest anti-microbial strategies are needed. This study evaluated the dose × time effects on antimicrobial action of ozone (O3) gas on the the Gram-negative Escherichia coli O157:H7 and Gram-positive Listeria monocytogenes, which are common contaminants in fresh produce. The study on a non-host tomato environment correlated the dose × time aspects of xenobiosis by examining the correlation between bacterial survival in terms of log-reduction and responses at the level of gene expression. In E. coli O157:H7, low (1 µg O3/g of fruit) and moderate (2 µg O3/g of fruit) doses caused insignificant reduction in survival, while a high dose (3 µg/g of fruit) caused significant reduction in survival in a time-dependent manner. In L. monocyt

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JO - PLoS ONE

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Responses of Escherichia coli and Listeria monocytogenes to ozone on non-host tomato: Efficacy of intervention and evidence of induced acclimation

Abstract

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