@article{837222be2852438da70a1af99b0c0c00,
title = "Relationships within the Calomys callosus species group based on amplified fragment length polymorphisms",
abstract = "Calomys callosus was identified almost 40 years ago as the rodent reservoir of Machupo virus (MACV, Arenaviridae), which causes Bolivian hemorrhagic fever (BHF), a disease endemic to northeastern Bolivia. However, the range of C. callosus s. l. far exceeds the known distribution of MACV and BHF. Four sampling regions representing different mitochondrial lineages within the C. callosus species group and an outgroup were evaluated for their genetic relationships using amplified fragment length polymorphisms (AFLP). Four AFLP primer combinations generated 596 bands, which were used for phylogenetic and population analyses. We show, using nuclear genetic markers, that the populations of rodents responsible for the maintenance and transmission of MACV are an independent monophyletic lineage, different from other lineages in other areas of Bolivia and South America. These data support the conclusions reached using mitochondrial DNA sequence from the cytochrome b and control region (D-loop) genes.",
keywords = "Amplified fragment length polymorphisms, Arenaviridae, Beni Department, Bolivia, Bolivian hemorrhagic fever, Calomys, Machupo virus, Systematics",
author = "Dragoo, {J. W.} and J. Salazar-Bravo and Layne, {L. J.} and Yates, {T. L.}",
note = "Funding Information: This research was supported by a grant from NIH (5-P01-AI39780). Dr S. Nichol (CDC) provided DNA from specimens infected with MACV from El Beni Department. Specimens from Bolivia were obtained through grants BSR 9015454, BSR 89200617, INT 9212839, from the National Science Foundation (NSF) to Dr Terry L. Yates. Specimens from Argentina were collected and provided by Dr Michael Mares, through NSF grant BSR-8906665, and National Geographic Society 4820-92 to M.A. Mares. Data were collected in the Molecular Biology Facility at the Department of Biology, University of New Mexico with assistance from George Rosenberg and Wade Wilson. We thank Michael Mares, Janet Braun, Robert Baker, and Cheryl Parmenter for providing us with frozen tissue samples. William C. Black, IV graciously provided access to source code for his rapdfst program. David S. Tinnin and G{\'a}bor R{\'a}cz were kind enough to read a draft of this manuscript. Special thanks go to Rodney L. Honeycutt and Holly Wichman, who provided constructive criticism during an oral presentation of these data. Two anonymous reviewers also helped to make this a better manuscript. ",
year = "2003",
month = jul,
doi = "10.1016/S0305-1978(02)00230-2",
language = "English",
volume = "31",
pages = "703--713",
journal = "Biochemical Systematics and Ecology",
issn = "0305-1978",
number = "7",
}