TY - JOUR
T1 - Regulation of pancreatic somatostatin gene expression by insulin and glucagon
AU - Ehrman, Melissa M.
AU - Melroe, Gregory T.
AU - Kittilson, Jeffrey D.
AU - Sheridan, Mark A.
N1 - Funding Information:
We would like to thank Leslie Alexander, Jean Boser, Miranda Huseth, Craig Moore, Lindsey Norbeck, Bart Slagter, and Nicole Very for their assistance with these experiments and the preparation of the manuscript. This research was supported by a grant from the National Science Foundation, U.S.A. (IBN 00764616) to MAS.
PY - 2005/5/12
Y1 - 2005/5/12
N2 - Rainbow trout were used as a model system to study the effects of insulin and glucagon on the expression of preprosomatostatins (PPSS). We previously showed that the endocrine pancreas of trout contains three mRNAs that encode for distinct somatostatin-containing peptides: PPSS I, which contains somatostain-14 (SS-14) at its C-terminus, and two separate PPSS IIs, PPSS II′ and PPSS II″, each containing [Tyr7, Gly 10]-SS-14 at their C-terminus. Rainbow trout injected (100 ng/g body weight) with insulin displayed elevated expression of PPSS II′ and PPSS II″ mRNAs. Glucagon-injected (100 ng/g body weight) animals displayed elevated pancreatic expression of all PPSS mRNAs compared to saline-injected control animals. Insulin directly stimulated the expression of pancreatic PPSS II′ and PPSS II″ mRNAs in vitro in a dose-dependent manner in the presence of 4 mM glucose. Glucagon, in the presence of 10 mM glucose, directly stimulated the expression of all PPSS mRNAs in a dose-dependent manner in vitro. These results indicate that the pancreatic expression of PPSS mRNAs is differentially regulated by insulin and glucagon and that the regulatory pattern is dependent on glucose concentration.
AB - Rainbow trout were used as a model system to study the effects of insulin and glucagon on the expression of preprosomatostatins (PPSS). We previously showed that the endocrine pancreas of trout contains three mRNAs that encode for distinct somatostatin-containing peptides: PPSS I, which contains somatostain-14 (SS-14) at its C-terminus, and two separate PPSS IIs, PPSS II′ and PPSS II″, each containing [Tyr7, Gly 10]-SS-14 at their C-terminus. Rainbow trout injected (100 ng/g body weight) with insulin displayed elevated expression of PPSS II′ and PPSS II″ mRNAs. Glucagon-injected (100 ng/g body weight) animals displayed elevated pancreatic expression of all PPSS mRNAs compared to saline-injected control animals. Insulin directly stimulated the expression of pancreatic PPSS II′ and PPSS II″ mRNAs in vitro in a dose-dependent manner in the presence of 4 mM glucose. Glucagon, in the presence of 10 mM glucose, directly stimulated the expression of all PPSS mRNAs in a dose-dependent manner in vitro. These results indicate that the pancreatic expression of PPSS mRNAs is differentially regulated by insulin and glucagon and that the regulatory pattern is dependent on glucose concentration.
KW - Glucagons
KW - Insulin
KW - Preprosomatostatin expression
KW - Rainbow trout
UR - http://www.scopus.com/inward/record.url?scp=18144406125&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2005.02.002
DO - 10.1016/j.mce.2005.02.002
M3 - Article
C2 - 15866425
AN - SCOPUS:18144406125
VL - 235
SP - 31
EP - 37
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -