TY - JOUR
T1 - Redundant and distinct functions of the ABA response loci ABA-insensitive(ABI)5 and ABRE-binding factor (ABF)3
AU - Finkelstein, Ruth
AU - Gampala, Srinivas S.L.
AU - Lynch, Tim J.
AU - Thomas, Terry L.
AU - Rock, Christopher D.
N1 - Funding Information:
We thank M. Thomashow for the cor78 cDNA, M. Delseny for the AtEm1 and RAB18 cDNAs, and I.Brocard and E. Zhuang for technical assistance. We thank the ABRC team at Ohio State University for efficient distribution of the T-DNA insertion lines from the SALK SIGnAL collection and the TILLING lines. This work was supported by National Science Foundation Grant IBN-9982779 to RRF, a 2003 Texas Tech University College of Arts and Sciences Research Enhancement Award to CDR, and USDA/NRI/CGP 01-35304-10940 to TLT. Funding for the TILLING and SIGnAL indexed insertion mutant collections was provided by the National Science Foundation.
PY - 2005/9
Y1 - 2005/9
N2 - Abscisic acid-responsive gene expression is regulated by numerous transcription factors, including a subgroup of basic leucine zipper factors that bind to the conserved cis-acting sequences known as ABA-responsive elements. Although one of these factors, ABA-insensitive 5 (ABI5), was identified genetically, the paucity of genetic data for the other family members has left it unclear whether they perform unique functions or act redundantly to ABI5 or each other. To test for potential redundancy with ABI5, we identified the family members with most similar effects and interactions in transient expression systems (ABF3 and ABF1), then characterized loss-of-function lines for those loci. The abf1 and abf3 monogenic mutant lines had at most minimal effects on germination or seed-specific gene expression, but the enhanced ABA- and stress-resistance of abf3 abi5 double mutants revealed redundant action of these genes in multiple stress responses of seeds and seedlings. Although ABI5, ABF3, and ABF1 have some overlapping effects, they appear to antagonistically regulate each other's expression at specific stages. Consequently, loss of any one factor may be partially compensated by increased expression of other family members.
AB - Abscisic acid-responsive gene expression is regulated by numerous transcription factors, including a subgroup of basic leucine zipper factors that bind to the conserved cis-acting sequences known as ABA-responsive elements. Although one of these factors, ABA-insensitive 5 (ABI5), was identified genetically, the paucity of genetic data for the other family members has left it unclear whether they perform unique functions or act redundantly to ABI5 or each other. To test for potential redundancy with ABI5, we identified the family members with most similar effects and interactions in transient expression systems (ABF3 and ABF1), then characterized loss-of-function lines for those loci. The abf1 and abf3 monogenic mutant lines had at most minimal effects on germination or seed-specific gene expression, but the enhanced ABA- and stress-resistance of abf3 abi5 double mutants revealed redundant action of these genes in multiple stress responses of seeds and seedlings. Although ABI5, ABF3, and ABF1 have some overlapping effects, they appear to antagonistically regulate each other's expression at specific stages. Consequently, loss of any one factor may be partially compensated by increased expression of other family members.
KW - ABA response element binding factor
KW - ABA-responsive gene expression
KW - ABI5
KW - Abscisic acid
KW - Arabidopsis
KW - Germination
UR - http://www.scopus.com/inward/record.url?scp=27144451538&partnerID=8YFLogxK
U2 - 10.1007/s11103-005-8767-2
DO - 10.1007/s11103-005-8767-2
M3 - Article
C2 - 16247556
AN - SCOPUS:27144451538
SN - 0167-4412
VL - 59
SP - 253
EP - 267
JO - Plant molecular biology
JF - Plant molecular biology
IS - 2
ER -