Recent advances in mass spectrometric analysis of glycoproteins

Alireza Banazadeh, Lucas Veillon, Kerry M. Wooding, Masoud Zabet-moghaddam, Yehia Mechref

Research output: Contribution to journalReview articlepeer-review

39 Scopus citations

Abstract

Glycosylation is one of the most common posttranslational modifications of proteins that plays essential roles in various biological processes, including protein folding, host–pathogen interaction, immune response, and inflammation and aberrant protein glycosylation is a well-known event in various disease states including cancer. As a result, it is critical to develop rapid and sensitive methods for the analysis of abnormal glycoproteins associated with diseases. Mass spectrometry (MS) in conjunction with different separation methods, such as capillary electrophoresis (CE), ion mobility (IM), and high performance liquid chromatography (HPLC) has become a popular tool for glycoprotein analysis, providing highly informative fragments for structural identification of glycoproteins. This review provides an overview of the developments and accomplishments in the field of glycomics and glycoproteomics reported between 2014 and 2016.

Original languageEnglish
Pages (from-to)162-189
Number of pages28
JournalELECTROPHORESIS
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2017

Keywords

  • Biomedical applications
  • Enrichment
  • Glycomics
  • Glycoproteomics
  • Mass spectrometry
  • Purification

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