Abstract
The charging of mammalian cell plasma membranes in response to ultrashort pulsed electric fields of 60 ns and field strengths up to 100 kV/cm was investigated. Membranes of Jurkat cells were stained with a potential-sensitive dye, Annine-6 and placed in a microreactor mounted on an inverted fluorescence microscope. Images of changes in the fluorescence intensity during the exposure were recorded with a high-sensitivity CCD-camera. A temporal resolution of 5 ns was achieved by illuminating the cells with a 5 ns laser pulse from a dye-laser. The laser pulse was synchronized with the high voltage pulse to record images at specific times before, during and after exposure to the electric field. When exposing Jurkat cells to a 60 ns, 100 kV/cm pulse, each hemisphere of the plasma membrane (as oriented with respect to the electrodes) responded uniquely to the applied field. From these observations it is possible to draw conclusions on the charging time of the membrane, maximum transmembrane voltages and the onset of poration.
Original language | English |
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Article number | 48 |
Pages (from-to) | 270-276 |
Number of pages | 7 |
Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
Volume | 5692 |
DOIs | |
State | Published - 2005 |
Event | Advanced Biomedical and Clinical Diagnostic Systems III - San Jose, CA, United States Duration: Jan 23 2005 → Jan 26 2005 |
Keywords
- Membrane potential
- Nanosecond pulsed electric fields
- Real-time fluorescence imaging