Differential mobility cytometry (DMC) has recently been established as a powerful method to capture cells and study adhesion processes. DMC uses an oscillation system and cell affinity chromatography to monitor cells as they adhere to a surface. In the past, differential images had to be created individually which limited the throughput of the method. A new method to create differential images is presented. The method involves the subtraction of short movies from each other to create a stack of differential images that can be easily analyzed. In the future, this method will make DMC more accessible and improve throughput.
- Cell adhesion
- Cell affinity chromatography
- Differential mobility cytometry