Previously, we isolated a 624-bp cDNA encoding for a 115-amino acid preprosomatostatin containing [Tyr7,Gly10]somatostatin (SS)-14 (now designated PPSS-II') obtained from the endocrine pancreas (Brockmann bodies) of rainbow trout. In this study we have characterized a second cDNA obtained from trout pancreas that is 600-bp in length and encodes for a 111-amino acid precursor containing [Tyr7,Gly10]SS-14 (PPSS-II'). The nucleotide and amino acid identity between the two cDNAs is 82.3 and 80.5%, respectively. Both PPSS-II' and PPSS-II' mRNA were present in esophagus, pyloric ceca, stomach, upper and lower intestine, and pancreas, whereas only SS-II' mRNA was present in brain. PPSS-II' mRNA was more abundant than PPSS-II' mRNA in pancreas, whereas PPSS-II' mRNA was more abundant than PPSS-II' mRNA in stomach. Fasting increased pancreatic PPSS-II' mRNA levels but had no effect on the levels of PPSS-II' mRNA. These results indicate the existence of two nonallelic pancreatic SS-II genes that are differentially expressed, both in terms of distribution among tissues and in terms of relative abundance within the tissues.
|Journal||American Journal of Physiology - Regulatory Integrative and Comparative Physiology|
|Issue number||6 46-6|
|State||Published - Dec 1999|
- Pancreatic somatostatin-II gene
- Preprosomatostatin- II