Rainbow trout (Oncorhynchus mykiss) possess two somatostatin mRNAs that are differentially expressed

Craig A. Moore, Jeffrey D. Kittilson, Melissa M. Ehrman, Mark A. Sheridan

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Previously, we isolated a 624-bp cDNA encoding for a 115-amino acid preprosomatostatin containing [Tyr7,Gly10]somatostatin (SS)-14 (now designated PPSS-II') obtained from the endocrine pancreas (Brockmann bodies) of rainbow trout. In this study we have characterized a second cDNA obtained from trout pancreas that is 600-bp in length and encodes for a 111-amino acid precursor containing [Tyr7,Gly10]SS-14 (PPSS-II'). The nucleotide and amino acid identity between the two cDNAs is 82.3 and 80.5%, respectively. Both PPSS-II' and PPSS-II' mRNA were present in esophagus, pyloric ceca, stomach, upper and lower intestine, and pancreas, whereas only SS-II' mRNA was present in brain. PPSS-II' mRNA was more abundant than PPSS-II' mRNA in pancreas, whereas PPSS-II' mRNA was more abundant than PPSS-II' mRNA in stomach. Fasting increased pancreatic PPSS-II' mRNA levels but had no effect on the levels of PPSS-II' mRNA. These results indicate the existence of two nonallelic pancreatic SS-II genes that are differentially expressed, both in terms of distribution among tissues and in terms of relative abundance within the tissues.

Original languageEnglish
Pages (from-to)R1553-R1561
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume277
Issue number6 46-6
DOIs
StatePublished - Dec 1999

Keywords

  • Pancreatic somatostatin-II gene
  • Preprosomatostatin- II
  • Preprosomatostatin-I

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