TY - JOUR
T1 - Rainbow trout (Oncorhynchus mykiss) possess two somatostatin mRNAs that are differentially expressed
AU - Moore, Craig A.
AU - Kittilson, Jeffrey D.
AU - Ehrman, Melissa M.
AU - Sheridan, Mark A.
PY - 1999/12
Y1 - 1999/12
N2 - Previously, we isolated a 624-bp cDNA encoding for a 115-amino acid preprosomatostatin containing [Tyr7,Gly10]somatostatin (SS)-14 (now designated PPSS-II') obtained from the endocrine pancreas (Brockmann bodies) of rainbow trout. In this study we have characterized a second cDNA obtained from trout pancreas that is 600-bp in length and encodes for a 111-amino acid precursor containing [Tyr7,Gly10]SS-14 (PPSS-II'). The nucleotide and amino acid identity between the two cDNAs is 82.3 and 80.5%, respectively. Both PPSS-II' and PPSS-II' mRNA were present in esophagus, pyloric ceca, stomach, upper and lower intestine, and pancreas, whereas only SS-II' mRNA was present in brain. PPSS-II' mRNA was more abundant than PPSS-II' mRNA in pancreas, whereas PPSS-II' mRNA was more abundant than PPSS-II' mRNA in stomach. Fasting increased pancreatic PPSS-II' mRNA levels but had no effect on the levels of PPSS-II' mRNA. These results indicate the existence of two nonallelic pancreatic SS-II genes that are differentially expressed, both in terms of distribution among tissues and in terms of relative abundance within the tissues.
AB - Previously, we isolated a 624-bp cDNA encoding for a 115-amino acid preprosomatostatin containing [Tyr7,Gly10]somatostatin (SS)-14 (now designated PPSS-II') obtained from the endocrine pancreas (Brockmann bodies) of rainbow trout. In this study we have characterized a second cDNA obtained from trout pancreas that is 600-bp in length and encodes for a 111-amino acid precursor containing [Tyr7,Gly10]SS-14 (PPSS-II'). The nucleotide and amino acid identity between the two cDNAs is 82.3 and 80.5%, respectively. Both PPSS-II' and PPSS-II' mRNA were present in esophagus, pyloric ceca, stomach, upper and lower intestine, and pancreas, whereas only SS-II' mRNA was present in brain. PPSS-II' mRNA was more abundant than PPSS-II' mRNA in pancreas, whereas PPSS-II' mRNA was more abundant than PPSS-II' mRNA in stomach. Fasting increased pancreatic PPSS-II' mRNA levels but had no effect on the levels of PPSS-II' mRNA. These results indicate the existence of two nonallelic pancreatic SS-II genes that are differentially expressed, both in terms of distribution among tissues and in terms of relative abundance within the tissues.
KW - Pancreatic somatostatin-II gene
KW - Preprosomatostatin- II
KW - Preprosomatostatin-I
UR - http://www.scopus.com/inward/record.url?scp=0033387540&partnerID=8YFLogxK
U2 - 10.1152/ajpregu.1999.277.6.r1553
DO - 10.1152/ajpregu.1999.277.6.r1553
M3 - Article
C2 - 10600899
AN - SCOPUS:0033387540
VL - 277
SP - R1553-R1561
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
SN - 0363-6119
IS - 6 46-6
ER -