TY - JOUR
T1 - Rainbow trout (Oncorhynchus mykiss) possess two insulin-encoding mRNAs that are differentially expressed
AU - Caruso, Michael A.
AU - Kittilson, Jeffery D.
AU - Raine, Jason
AU - Sheridan, Mark A.
N1 - Funding Information:
We thank Patrick Blaufuss, Sarah Klein, Felicia Lamb, Heidi Malkuch, Lindsey Norbeck, and Chad Walock for their help in conducting the experiments and Rian Lee for his technical support with automated sequencing. We also thank the staff of the Alma Aquaculture Research Station for providing and maintaining the embryos. The full open reading frames for both catfish insulin genes was generously provided by Dr. Geoffrey C, Waldbieser (USDA-ARS-Catfish Genetics Research Unit, Stoneville, MS) for the purpose of sequence similarity analysis. This work was supported by grants from the National Science Foundation (IOB 0444860, EPS 0132289) to M.A.S. J.R. was supported by a fellowship from the Department of Biomedical Sciences, University of Guelph.
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Insulin (INS) plays a critical role in the growth, development, and metabolism of vertebrates. In this study, two unique cDNAs that encode preproinsulin were isolated, cloned and sequenced from the endocrine pancreas (Brockmann body) of rainbow trout. One 592-bp cDNA (INS 1) encodes a 105-amino acid protein and the other 587-bp cDNA (INS 2) encodes a 107-amino acid protein. The sequences share 93% nucleotide identity and 91.4% deduced amino acid identity. Quantitative real-time PCR revealed that the two INS-encoding mRNAs were differentially expressed, both in terms of distribution among tissues as well as in terms of abundance within selected tissues of juvenile trout. Both INS 1 and INS 2 mRNAs were detected in pancreas, adipose tissue, pyloric cecum, and brain; however, only INS 1 mRNA was detected in upper and lower intestine and pituitary. In all cases where INS 1 and INS 2 were co-expressed, INS 1 was more abundant. INS 1 and INS 2 also were differentially expressed in various body regions (head, body, and tail) during embryonic development. Both INS 1 and INS 2 mRNAs were detected early in development (29 days post-fertilization), but their expression declined as development proceeded (through 90 days post-fertilization); in most cases, unlike the situation in juveniles, INS 2 mRNA was more abundant than INS 1 mRNA in embryos. These findings contribute to our understanding of the evolution, distribution, and function of INS.
AB - Insulin (INS) plays a critical role in the growth, development, and metabolism of vertebrates. In this study, two unique cDNAs that encode preproinsulin were isolated, cloned and sequenced from the endocrine pancreas (Brockmann body) of rainbow trout. One 592-bp cDNA (INS 1) encodes a 105-amino acid protein and the other 587-bp cDNA (INS 2) encodes a 107-amino acid protein. The sequences share 93% nucleotide identity and 91.4% deduced amino acid identity. Quantitative real-time PCR revealed that the two INS-encoding mRNAs were differentially expressed, both in terms of distribution among tissues as well as in terms of abundance within selected tissues of juvenile trout. Both INS 1 and INS 2 mRNAs were detected in pancreas, adipose tissue, pyloric cecum, and brain; however, only INS 1 mRNA was detected in upper and lower intestine and pituitary. In all cases where INS 1 and INS 2 were co-expressed, INS 1 was more abundant. INS 1 and INS 2 also were differentially expressed in various body regions (head, body, and tail) during embryonic development. Both INS 1 and INS 2 mRNAs were detected early in development (29 days post-fertilization), but their expression declined as development proceeded (through 90 days post-fertilization); in most cases, unlike the situation in juveniles, INS 2 mRNA was more abundant than INS 1 mRNA in embryos. These findings contribute to our understanding of the evolution, distribution, and function of INS.
KW - Insulin evolution
KW - Insulin gene expression
KW - Real-time PCR
UR - http://www.scopus.com/inward/record.url?scp=38849162869&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2007.09.006
DO - 10.1016/j.ygcen.2007.09.006
M3 - Article
C2 - 17963757
AN - SCOPUS:38849162869
SN - 0016-6480
VL - 155
SP - 695
EP - 704
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 3
ER -