Quantitative glycomics strategies

Yehia Mechref, Yunli Hu, Janie L. Desantos-Garcia, Ahmed Hussein, Haixu Tang

Research output: Contribution to journalReview articlepeer-review

57 Scopus citations

Abstract

The correlations between protein glycosylation and many biological processes and diseases are increasing the demand for quantitative glycomics strategies enabling sensitive monitoring of changes in the abundance and structure of glycans. This is currently attained through multiple strategies employing several analytical techniques such as capillary electrophoresis, liquid chromatography, and mass spectrometry. The detection and quantification of glycans often involve labeling with ionic and/or hydrophobic reagents. This step is needed in order to enhance detection in spectroscopic and mass spectrometric measurements. Recently, labeling with stable isotopic reagents has also been presented as a very viable strategy enabling relative quantitation. The different strategies available for reliable and sensitive quantitative glycomics are herein described and discussed.

Original languageEnglish
Pages (from-to)874-884
Number of pages11
JournalMolecular and Cellular Proteomics
Volume12
Issue number4
DOIs
StatePublished - Apr 2013

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