Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach

L. Veillon, S. Zhou, Y. Mechref

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Glycosylation is one of the most common and essential protein modifications. Glycans conjugated to biomolecules modulate the function of such molecules through both direct recognition of glycan structures and indirect mechanisms that involve the control of protein turnover rates, stability, and conformation. The biological attributes of glycans in numerous biological processes and implications in a number of diseases highlight the necessity for comprehensive characterization of protein glycosylation. This chapter reviews cutting-edge methods and tools developed to facilitate quantitative glycomics. This chapter highlights the different methods employed for the release and purification of glycans from biological samples. The most effective labeling methods developed for sensitive quantitative glycomics are also described and discussed. The chromatographic approaches that have been used effectively in glycomics are also highlighted.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages431-477
Number of pages47
DOIs
StatePublished - 2017

Publication series

NameMethods in Enzymology
Volume585
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Chromatographic methods
  • Glycomics
  • Labeling of glycans
  • Purification
  • Release of glycans

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    Veillon, L., Zhou, S., & Mechref, Y. (2017). Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach. In Methods in Enzymology (pp. 431-477). (Methods in Enzymology; Vol. 585). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2016.11.006