Quantitative chiral analysis of salsolinol in different brain regions of rats genetically predisposed to alcoholism

Tatiana Rojkovicova, Yehia Mechref, Jason A. Starkey, Guangxiang Wu, Richard L. Bell, William J. McBride, Milos V. Novotny

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

A method to determine the catecholamine content in putamen (CPU) and midbrain (MB) regions of the brain of alcohol-preferring rats (P) is presented with a focus on the low-level detection of S,R-salsolinol, a metabolite of dopamine and a putative alcoholism marker. The developed strategy allows both quantitative profiling of related catecholamines and the enantiomeric separation and quantification of the S- and R-salsolinol isomers and their ratios. The described LC/MS strategy simplifies the current methodology that typically employs GC-MS by eliminating the need for derivatization. The data also suggest an increase in the non-enzymatic formation of salsolinol as a consequence of ethanol exposure.

Original languageEnglish
Pages (from-to)206-214
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume863
Issue number2
DOIs
StatePublished - Mar 1 2008

Keywords

  • Alcoholism
  • Catecholamines
  • Chiral separations
  • Multiple reaction monitoring (MRM)
  • Salsolinol
  • Tandem mass spectrometry

Fingerprint Dive into the research topics of 'Quantitative chiral analysis of salsolinol in different brain regions of rats genetically predisposed to alcoholism'. Together they form a unique fingerprint.

  • Cite this