Qualitative and Quantitative Drug residue analyses: Florfenicol in white-tailed deer (Odocoileus virginianus) and supermarket meat by liquid chromatography tandem-mass spectrometry

Shanoy C. Anderson, Seenivasan Subbiah, Angella Gentles, Galen Austin, Paul Stonum, Tiffanie A. Brooks, Chance Brooks, Ernest E. Smith

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A method for confirmation and detection of Florfenicol amine residues in white-tailed deer tissues was developed and validated in our laboratory. Tissue samples were extracted with ethyl acetate and cleaned up on sorbent (Chem-elut) cartridges. Liguid chromatography (LC) separation was achieved on a Zorbax Eclipse plus C18 column with gradient elution using a mobile phase composed of ammonium acetate in water and methanol at a flow rate of 300 μL/min. Qualitative and quantitative analyses were carried out using liquid chromatography − heated electrospray ionization(HESI) and atmospheric pressure chemical ionization (APCI)-tandem mass spectrometry in the multiple reaction monitoring (MRM) interface. The limits of detection (LODs) for HESI and APCI probe were 1.8 ng/g and 1.4 ng/g respectively. Limits of quantitation (LOQs) for HESI and APCI probe were 5.8 ng/g and 3.4 ng/g respectively. Mean recovery values ranged from 79% to 111% for APCI and 30% to 60% for HESI. The validated method was used to determine white-tailed deer florfenicol tissue residue concentration 10-days after exposure. Florfenicol tissue residues concentration ranged from 0.4 to 0.6 μg/g for liver and 0.02-0.05 μg/g for muscle and a trace in blood samples. The concentration found in the tested edible tissues were lower than the maximum residual limit (MRL) values established by the federal drug administration (FDA) for bovine tissues. In summary, the resulting optimization procedures using the sensitivity of HESI and APCI probes in the determination of florfenicol in white-tailed deer tissue are the most compelling conclusions in this study, to the extent that we have applied this method in the evaluation of supermarket samples drug residue levels as a proof of principle.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1033-1034
DOIs
StatePublished - Oct 15 2016

Keywords

  • Chromatography
  • Drug residue florfenicol amine
  • Florfenicol
  • LC–MS/MS
  • White-tailed deer

Fingerprint Dive into the research topics of 'Qualitative and Quantitative Drug residue analyses: Florfenicol in white-tailed deer (Odocoileus virginianus) and supermarket meat by liquid chromatography tandem-mass spectrometry'. Together they form a unique fingerprint.

Cite this