Purification and characterization of hepatic triacylglycerol lipase isolated from rainbow trout, Oncorhynchus mykiss

Jamie S. Harmon, Kim G. Michelsen, Mark A. Sheridan

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Trialcylglycerol (TG) lipase was isolated and partially purified from rainbow trout liver. Triacylglycerol lipase activity was assayed by measuring14C-oleic acid release from14C-triolein.14C-oleic acid release was linear for up to two hours. Optimal activity occurred at pH 7.0 and 15°C. Most of the lipase activity was recovered in the cytosolic fraction. A 27,000-fold purification was achieved after Sepharose (Bio-gel A 0.5 M, 200-400 mesh) chromatography of a resuspended 20% ammonium sulfate fraction. The molecular weight of the trout hepatic lipase as determined by size-exclusion chromatography and by SDS-polyacrylamide gel electrophoresis was 40-43 kD. Lipase-mediated hydrolysis of TG resulted in the production of diacylglycerols, monoacylglycerols, and fatty acids. Kinetic analysis indicated that Vmax=0.016 nmol/h/mg protein and that Km=0.28 mM triolein. Lipolytic activity was enhanced in the presence of cAMP/ATP-Mg2+. These results suggest that the liver of trout possesses a neutral TG lipase that is responsible for mobilizing stored TG and is catalytically activated by phosphorylation.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalFish Physiology and Biochemistry
Volume9
Issue number4
DOIs
StatePublished - Dec 1991

Keywords

  • Oncorhynchus mykiss
  • lipolysis
  • liver
  • rainbow trout
  • triacylglycerol lipase

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