Proteomic and physiological responses of leopard sharks Triakis semifasciata to salinity change.

W Wesley Dowd, Breanna Harris, Joseph J Cech, Dietmar Kultz

Research output: Contribution to journalArticle

Abstract

Partially euryhaline elasmobranchs may tolerate physiologically challenging, variable salinity conditions in estuaries as a tradeoff to reduce predation risk or to gain access to abundant food resources. To further understand these trade-offs and to evaluate the underlying mechanisms, we examined the responses of juvenile leopard sharks to salinity changes using a suite of measurements at multiple organizational levels: gill and rectal gland proteomes (using 2-D gel electrophoresis and tandem mass spectrometry), tissue biochemistry (Na+/K+-ATPase, caspase 3/7 and chymotrypsin-like proteasome activities), organismal physiology (hematology, plasma composition, muscle moisture) and individual behavior. Our proteomics results reveal coordinated molecular responses to low salinity – several of which are common to both rectal gland and gill – including changes in amino acid and inositol (i.e. osmolyte) metabolism, energy metabolism and proteins related to transcription, translation and prot
Original languageEnglish
Pages (from-to)210-224
JournalJournal of Experimental Biology
StatePublished - 2010

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