Profile of the bovine acute-phase response following an intravenous bolus-dose lipopolysaccharide challenge

Jeffery A. Carroll, Richard R. Reuter, Chadwick C. Chase, Samuel W. Coleman, David G. Riley, Donald E. Spiers, John D. Arthington, Michael L. Galyean

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Our objective was to characterize further the acute-phase response following endotoxin (i.e. lipopolysaccharide; LPS) exposure in the bovine. Nine pure-bred Angus castrated males (i.e. steers; average body weight = 299 ± 5 kg) were used in a randomized complete block design in environmentally controlled chambers, set at thermoneutral level, to characterize the acute physiological, endocrine, immune, and acute-phase protein responses following an i.v. bolus administration of 2.5 μg of LPS/kg body weight. One day before administration of LPS, all steers were fitted with an indwelling jugular vein catheter for serial blood collection. Blood samples were collected at 30-min intervals from -2 h to 8 h relative to the LPS challenge (time 0), and serum was harvested and stored at -80°C until analyzed for concentrations of cortisol, pro-inflammatory cytokines, and acute-phase proteins. Indicators of thermal status (i.e. rectal temperature, ruminal temperature, respiration rate, sweat rate, and skin temperatures) were measured at 30-min intervals from -1 h to 6 h relative to the challenge. Endotoxin exposure increased (P < 0.05) serum concentrations of cortisol, tumor necrosis factor-α (TNF-α), interleukin 1-β (IL-1β), IL-6, interferon-γ (IFN-γ), and serum amyloid A. Respiration rate, rectal temperature, and rump skin temperature also were increased (P < 0.05) following LPS administration. Endotoxin exposure dramatically decreased ear skin temperature (P = 0.002), but tended to increase (P < 0.10) ruminal temperature, shoulder skin temperature, and shoulder sweat rate. Serum concentrations of acid soluble protein, α-acid glycoprotein, IL-4 and IL-2, and rump sweat rate were not altered (P > 0.24) by the challenge. To our knowledge, this report is the most complete characterization of the bovine acute-phase response to a bolus-dose endotoxin challenge conducted under thermoneutral conditions and should provide foundation data for future research.

Original languageEnglish
Pages (from-to)81-89
Number of pages9
JournalInnate Immunity
Volume15
Issue number2
DOIs
StatePublished - 2009

Keywords

  • Acute-phase proteins
  • Acute-phase response
  • Bovine
  • Cytokines
  • Lipopolysaccharide

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    Carroll, J. A., Reuter, R. R., Chase, C. C., Coleman, S. W., Riley, D. G., Spiers, D. E., Arthington, J. D., & Galyean, M. L. (2009). Profile of the bovine acute-phase response following an intravenous bolus-dose lipopolysaccharide challenge. Innate Immunity, 15(2), 81-89. https://doi.org/10.1177/1753425908099170