Production of hydrogen peroxide by Lactobacillus delbrueckii subsp. lactis as Influenced by media used for propagation of cells

Cells of Lactobacillus delbrueckii subsp. lactis I were grown in several media to determine the most suitable medium for production of H₂O₂ during refrigerated storage. H₂O₂ was detected from cells that were resuspended in phosphate buffer containing variable amounts of glucose. Cells grown in MRS broth produced more H₂O₂ during refrigerated storage than those grown in LBS pr PTM. Cells grown in LBS produced more H₂O₂ then those grown in PTM. For both MRS and LBS, cells inoculated in phosphate buffer containing 0% glucose produced significantly more H₂O₂ than those containing 1% or 10% glucose. MRS medium appears to be the best medium for growing cells for the production of H₂O₂.