Preparation of giant unilamellar vesicles from damp lipid film for better lipid compositional uniformity

Eda Baykal-Caglar, Ebrahim Hassan-Zadeh, Bahar Saremi, Juyang Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Giant unilamellar vesicles (GUVs) containing cholesterol often have a wide distribution in lipid composition. In this study, GUVs of 1,2-dioleoyl-sn- glycero-3-phosphocholine(DOPC)/1,2-distearoyl-sn-glycero-3-phosphocholine(DSPC)/ cholesterol and 1,2-diphytanoyl-sn-glycero-3-phosphocholine(diPhyPC)/1,2- dipalmitoyl-sn-glycero-3-phosphocholine(DPPC)/cholesterol were prepared from dry lipid films using the standard electroformation method as well as a modified method from damp lipid films, which are made from compositional uniform liposomes prepared using the Rapid Solvent Exchange (RSE) method. We quantified the lipid compositional distributions of GUV by measuring the miscibility transition temperature of GUVs using fluorescence microscopy, since a narrower distribution in the transition temperature should correspond to a more uniform distribution in GUV lipid composition. Cholesterol molecules can demix from other lipids in dry state and form cholesterol crystals. Using optical micro
Original languageEnglish
Pages (from-to)2598-2604
JournalBiochimica et Biophysica Acta - Biomembranes
StatePublished - Nov 1 2012

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