TY - JOUR
T1 - Preliminary evaluation of Prodigiosa herbal tea
T2 - cytotoxicity and GLUT2 expression in HepG2 cells
AU - Viñas, René
AU - Smith, Ernest E.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/4
Y1 - 2013/4
N2 - In this study, we characterized the potential cytotoxicity of Prodigiosa herbal tea on hepatocellular carcinoma (HepG2) cells in vitro. HepG2 were submitted to both concentration and time-dependent toxicity evaluation of the herbal tea of Prodigiosa (0-200 mg·L-1) following exposures of 24 and 48 h. Cell viability was determined with the (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the data reported relative to the non-treated cells. Cellular toxicity was observed at concentrations greater than 100 mg·L-1 of Prodigiosa with a clear indication of time-dependent decrease in cell viability. In contrast the 100 mg·L-1 significantly stimulated cell growth over the non-treated cells. The expression data indicated that Prodigiosa alters glucose transporter (GLUT2) protein and cDNA levels in HeG2 cells. The preliminary data from this study suggest that the effects from Prodigiosa herbal tea negatively influence HepG2 cell viability, GLUT2 protein, and mRNA expression at concentrations greater than 100 mg·L-1. Ongoing work in our laboratory will characterize the specific bioactive compound that is associated with the regulatory mechanisms of Prodigiosa on GLUT2 expression and the effects of Prodigiosa in normal liver cells.
AB - In this study, we characterized the potential cytotoxicity of Prodigiosa herbal tea on hepatocellular carcinoma (HepG2) cells in vitro. HepG2 were submitted to both concentration and time-dependent toxicity evaluation of the herbal tea of Prodigiosa (0-200 mg·L-1) following exposures of 24 and 48 h. Cell viability was determined with the (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the data reported relative to the non-treated cells. Cellular toxicity was observed at concentrations greater than 100 mg·L-1 of Prodigiosa with a clear indication of time-dependent decrease in cell viability. In contrast the 100 mg·L-1 significantly stimulated cell growth over the non-treated cells. The expression data indicated that Prodigiosa alters glucose transporter (GLUT2) protein and cDNA levels in HeG2 cells. The preliminary data from this study suggest that the effects from Prodigiosa herbal tea negatively influence HepG2 cell viability, GLUT2 protein, and mRNA expression at concentrations greater than 100 mg·L-1. Ongoing work in our laboratory will characterize the specific bioactive compound that is associated with the regulatory mechanisms of Prodigiosa on GLUT2 expression and the effects of Prodigiosa in normal liver cells.
KW - GLUT2
KW - HepG2 cells
KW - Prodigiosa cytotoxicity
KW - herbal tea Prodigiosa
UR - http://www.scopus.com/inward/record.url?scp=84879937502&partnerID=8YFLogxK
U2 - 10.1080/02772248.2013.801977
DO - 10.1080/02772248.2013.801977
M3 - Article
AN - SCOPUS:84879937502
VL - 95
SP - 669
EP - 678
JO - Toxicological and Environmental Chemistry
JF - Toxicological and Environmental Chemistry
SN - 0277-2248
IS - 4
ER -