Positively charged lysine at the N-terminus of the signal peptide of the Escherichia coli alkaline phosphatase provides the secretion efficiency and is involved in the interaction with anionic phospholipids

Marina A. Nesmeyanova, Andrew L. Karamyshev, Zemphyra N. Karamysheva, Andrey E. Kalinin, Vladimir N. Ksenzenko, Andrey V. Kajava

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Positively charged amino acid residues at the N-terminus of the signal peptide (SP) have been proposed to play a significant role in the initial step of protein secretion in bacteria. To test this hypothesis, Lys(-20) of the Escherichia coli alkaline phosphatase SP was replaced by other amino acid residues, and the effect of these substitutions on protein maturation was studied. The introduction of negatively charged and hydrophobic amino acids resulted in a decrease in secretion efficiency and impaired the SP-APL interaction, whereas His and Tyr had no significant effect. A structural analysis of the SP-APL interaction suggests that the positively charged Lyst(-20) determines the stability of the complex.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalFEBS Letters
Volume403
Issue number2
DOIs
StatePublished - Feb 17 1997

Keywords

  • Alkaline phosphatase
  • Anionic phospholipid
  • Escherichia coli
  • Protein secretion
  • Signal peptide
  • Stereochemical analysis

Fingerprint Dive into the research topics of 'Positively charged lysine at the N-terminus of the signal peptide of the Escherichia coli alkaline phosphatase provides the secretion efficiency and is involved in the interaction with anionic phospholipids'. Together they form a unique fingerprint.

  • Cite this