Porous polyacrylamide monoliths in hydrophilic interaction capillary electrochromatography of oligosaccharides

Vilém Guryča, Yehia Mechref, Anders K. Palm, Jiří Michálek, Věra Pacáková, Miloš V. Novotný

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Capillary electrochromatography (CEC) of oligosaccharides in porous polyacrylamide monoliths has been explored. While it is possible to alter separation capacity for various compounds by copolymerization of suitable separation ligands in the polymerization backbone, "blank" acrylamide matrix is also capable of sufficient resolution of oligosaccharides in the hydrophilic interaction mode. The "blank" acrylamide network, formed with a more rigid crosslinker, provides maximum efficiency for separations (routinely up to 350,000 theoretical plates/m for fluorescently-labeled oligosaccharides). These columns yield a high spatial resolution of the branched glycan isomers and large column permeabilities. From the structural point of view, some voids are observable in the monoliths at the mesoporous range (mean pore radius ca. 35 nm, surface area of 74 m2/g), as measured by intrusion porosimetry in the dry state.

Original languageEnglish
Pages (from-to)3-13
Number of pages11
JournalJournal of Biochemical and Biophysical Methods
Volume70
Issue number1
DOIs
StatePublished - Feb 23 2007

Keywords

  • Analytical glycobiology
  • Branched oligosaccharides
  • Capillary electrochromatography
  • Polyacrylamide monoliths

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