TY - JOUR
T1 - Porous polyacrylamide monoliths in hydrophilic interaction capillary electrochromatography of oligosaccharides
AU - Guryča, Vilém
AU - Mechref, Yehia
AU - Palm, Anders K.
AU - Michálek, Jiří
AU - Pacáková, Věra
AU - Novotný, Miloš V.
N1 - Funding Information:
We are indebted to the following organizations for their financial support: the Hlavka Foundation, Czech Republic; the Academy of Sciences of the Czech Republic (the project AVOZ 405000505); the Ministry of Education, Youth and Sports (the project 1M0538) and the National Institute of General Medical Sciences, U.S. Department of Health and Human Services (Grant No. GM24349). This work was also supported by the Indiana Genomics Initiative (INGEN), which is funded in part by the Lilly Endowment, Inc. (USA).
PY - 2007/2/23
Y1 - 2007/2/23
N2 - Capillary electrochromatography (CEC) of oligosaccharides in porous polyacrylamide monoliths has been explored. While it is possible to alter separation capacity for various compounds by copolymerization of suitable separation ligands in the polymerization backbone, "blank" acrylamide matrix is also capable of sufficient resolution of oligosaccharides in the hydrophilic interaction mode. The "blank" acrylamide network, formed with a more rigid crosslinker, provides maximum efficiency for separations (routinely up to 350,000 theoretical plates/m for fluorescently-labeled oligosaccharides). These columns yield a high spatial resolution of the branched glycan isomers and large column permeabilities. From the structural point of view, some voids are observable in the monoliths at the mesoporous range (mean pore radius ca. 35 nm, surface area of 74 m2/g), as measured by intrusion porosimetry in the dry state.
AB - Capillary electrochromatography (CEC) of oligosaccharides in porous polyacrylamide monoliths has been explored. While it is possible to alter separation capacity for various compounds by copolymerization of suitable separation ligands in the polymerization backbone, "blank" acrylamide matrix is also capable of sufficient resolution of oligosaccharides in the hydrophilic interaction mode. The "blank" acrylamide network, formed with a more rigid crosslinker, provides maximum efficiency for separations (routinely up to 350,000 theoretical plates/m for fluorescently-labeled oligosaccharides). These columns yield a high spatial resolution of the branched glycan isomers and large column permeabilities. From the structural point of view, some voids are observable in the monoliths at the mesoporous range (mean pore radius ca. 35 nm, surface area of 74 m2/g), as measured by intrusion porosimetry in the dry state.
KW - Analytical glycobiology
KW - Branched oligosaccharides
KW - Capillary electrochromatography
KW - Polyacrylamide monoliths
UR - http://www.scopus.com/inward/record.url?scp=33846888042&partnerID=8YFLogxK
U2 - 10.1016/j.jbbm.2006.11.002
DO - 10.1016/j.jbbm.2006.11.002
M3 - Article
C2 - 17254635
AN - SCOPUS:33846888042
SN - 0165-022X
VL - 70
SP - 3
EP - 13
JO - Journal of Biochemical and Biophysical Methods
JF - Journal of Biochemical and Biophysical Methods
IS - 1
ER -