TY - JOUR
T1 - Polygenic expression of somatostatin in rainbow trout, Oncorhynchus mykiss
T2 - Evidence of a preprosomatostatin encoding somatostatin-14
AU - Kittilson, Jeffrey D.
AU - Moore, Craig A.
AU - Sheridan, Mark A.
N1 - Funding Information:
Thanks go to Jayme Steig, Melissa Ehrman, Bart Slagter, Marty Pesek, and Yung-hsi Kao for technical assistance. Animals were provided by the North Dakota Game and Fish Department and the U.S. Department of the Interior, Fish and Wildlife Service (Garrison National Hatchery). This research was supported by a grant from the National Science Foundation, U.S.A. (IBN 9723058) to M.A.S.
PY - 1999/4
Y1 - 1999/4
N2 - Previously we reported the existence of two distinct cDNAs in rainbow trout that encode for separate preprosomatostatins (PPSS), each containing [Tyr7, Gly10]somatostatin-14. In the present study, we used rainbow trout to further characterize the polygenic origin of somatostatins (SSs), a peptide hormone important in the regulation of growth, development, and metabolism of vertebrates. A two-phase rapid amplification of cDNA ends (RACE)-PCR was used for the isolation of selected cDNAs. We amplified and sequenced a ca. 350-bp 3' RACE-PCR fragment. Based upon this sequence we designed a second gene-specific primer for 5' RACE-PCR which yielded a 452- bp fragment. Sequence analysis revealed a 745-bp cDNA containing the complete 5'-untranslated region, a single initiation site 118 bases from the most 5' end, and a single putative polyadenylation site 17 bases from the most 3' end that was terminated with a polyadenylated tail. The deduced protein is a 114- amino acid PPSS molecule that contains a number of putative processing sites, potentially yielding a 26-amino acid peptide that could be processed further to a 14-amino acid peptide identical in structure to mammalian SS-14. Northern analysis revealed that PPSS-I was expressed in the pancreas, stomach, intestine, and brain of rainbow trout. These results suggest a polygenic origin of SS, possibly resulting from gene duplication events prior to the divergence of teleosts.
AB - Previously we reported the existence of two distinct cDNAs in rainbow trout that encode for separate preprosomatostatins (PPSS), each containing [Tyr7, Gly10]somatostatin-14. In the present study, we used rainbow trout to further characterize the polygenic origin of somatostatins (SSs), a peptide hormone important in the regulation of growth, development, and metabolism of vertebrates. A two-phase rapid amplification of cDNA ends (RACE)-PCR was used for the isolation of selected cDNAs. We amplified and sequenced a ca. 350-bp 3' RACE-PCR fragment. Based upon this sequence we designed a second gene-specific primer for 5' RACE-PCR which yielded a 452- bp fragment. Sequence analysis revealed a 745-bp cDNA containing the complete 5'-untranslated region, a single initiation site 118 bases from the most 5' end, and a single putative polyadenylation site 17 bases from the most 3' end that was terminated with a polyadenylated tail. The deduced protein is a 114- amino acid PPSS molecule that contains a number of putative processing sites, potentially yielding a 26-amino acid peptide that could be processed further to a 14-amino acid peptide identical in structure to mammalian SS-14. Northern analysis revealed that PPSS-I was expressed in the pancreas, stomach, intestine, and brain of rainbow trout. These results suggest a polygenic origin of SS, possibly resulting from gene duplication events prior to the divergence of teleosts.
UR - http://www.scopus.com/inward/record.url?scp=0032937441&partnerID=8YFLogxK
U2 - 10.1006/gcen.1998.7238
DO - 10.1006/gcen.1998.7238
M3 - Article
C2 - 10094862
AN - SCOPUS:0032937441
SN - 0016-6480
VL - 114
SP - 88
EP - 96
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -