Place-based screening of mixtures of dominant emerging contaminants measured in Lake Michigan using zebrafish embryo gene expression assay.

Jordan Crago, Rebecca Klaper

Research output: Contribution to journalArticlepeer-review

Abstract

Determining impacts of emerging contaminants is difficult due to the different concentrations of mix- tures of these chemicals over a landscape. Assessment approaches need to account for absorption, dis- tribution, metabolism and excretion of the chemicals in an organism, and potential crosstalk between molecular pathways. The goal of this study was to assess the utility of employing a modified zebrafish embryo toxicity (ZFET) assay that assesses morphological alterations and measurements of estrogen- associated mRNA transcripts, to exposure of a mixtures of chemicals at concentrations measured in several locations in Lake Michigan. The 5 pharmaceuticals in this study were carbamazepine, diltiazem, fluoxetine, gemfibrozil and metformin. Exposures consisted of 4 concentrations of each individual chemical, mixture concentrations measured at seven locations in Lake Michigan, or 17b-estradiol. The relative expression of Estrogen Receptor-alpha, brain aromatase (CYP19A2), and gonadotropin
Original languageEnglish
Pages (from-to)1226-1234
JournalChemosphere
StatePublished - Jan 2018

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