Phytosterol biosynthesis: Isotope effects associated with biomethylation formation to 24-alkene sterol isomers

De an Guo, Zhonghua Jia, W. David Nes

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Changes in 24-alkene sterol product distribution resulting from deuterium substitution on the coenzyme methyl group of AdoMet and on the sterol acceptor molecule at C-23 and C-24 were used to determine kinetic isotope effects for the terminating deprotonations involved in sterol biomethylation catalyzed by (S)-adenosyl-L-methionine-Δ24-sterol methyl transferase (SMT) enzyme. By this method 24(28)-methylene cycloartanol and cyclosadol were shown to be synthesized by two different SMT enzymes.

Original languageEnglish
Pages (from-to)6823-6826
Number of pages4
JournalTetrahedron Letters
Volume37
Issue number38
DOIs
StatePublished - Sep 16 1996

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