Phytosterol biosynthesis: Isotope effects associated with biomethylation formation to 24-alkene sterol isomers

De an Guo; Zhonghua Jia; W. David Nes

doi:10.1016/0040-4039(96)01534-1

Phytosterol biosynthesis: Isotope effects associated with biomethylation formation to 24-alkene sterol isomers

De an Guo, Zhonghua Jia, W. David Nes

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Changes in 24-alkene sterol product distribution resulting from deuterium substitution on the coenzyme methyl group of AdoMet and on the sterol acceptor molecule at C-23 and C-24 were used to determine kinetic isotope effects for the terminating deprotonations involved in sterol biomethylation catalyzed by (S)-adenosyl-L-methionine-Δ²⁴-sterol methyl transferase (SMT) enzyme. By this method 24(28)-methylene cycloartanol and cyclosadol were shown to be synthesized by two different SMT enzymes.

Original language	English
Pages (from-to)	6823-6826
Number of pages	4
Journal	Tetrahedron Letters
Volume	37
Issue number	38
DOIs	https://doi.org/10.1016/0040-4039(96)01534-1
State	Published - Sep 16 1996

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title = "Phytosterol biosynthesis: Isotope effects associated with biomethylation formation to 24-alkene sterol isomers",

abstract = "Changes in 24-alkene sterol product distribution resulting from deuterium substitution on the coenzyme methyl group of AdoMet and on the sterol acceptor molecule at C-23 and C-24 were used to determine kinetic isotope effects for the terminating deprotonations involved in sterol biomethylation catalyzed by (S)-adenosyl-L-methionine-Δ24-sterol methyl transferase (SMT) enzyme. By this method 24(28)-methylene cycloartanol and cyclosadol were shown to be synthesized by two different SMT enzymes.",

author = "Guo, {De an} and Zhonghua Jia and Nes, {W. David}",

year = "1996",

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doi = "10.1016/0040-4039(96)01534-1",

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journal = "Tetrahedron Letters",

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TY - JOUR

T1 - Phytosterol biosynthesis

T2 - Isotope effects associated with biomethylation formation to 24-alkene sterol isomers

AU - Guo, De an

AU - Jia, Zhonghua

AU - Nes, W. David

PY - 1996/9/16

Y1 - 1996/9/16

N2 - Changes in 24-alkene sterol product distribution resulting from deuterium substitution on the coenzyme methyl group of AdoMet and on the sterol acceptor molecule at C-23 and C-24 were used to determine kinetic isotope effects for the terminating deprotonations involved in sterol biomethylation catalyzed by (S)-adenosyl-L-methionine-Δ24-sterol methyl transferase (SMT) enzyme. By this method 24(28)-methylene cycloartanol and cyclosadol were shown to be synthesized by two different SMT enzymes.

AB - Changes in 24-alkene sterol product distribution resulting from deuterium substitution on the coenzyme methyl group of AdoMet and on the sterol acceptor molecule at C-23 and C-24 were used to determine kinetic isotope effects for the terminating deprotonations involved in sterol biomethylation catalyzed by (S)-adenosyl-L-methionine-Δ24-sterol methyl transferase (SMT) enzyme. By this method 24(28)-methylene cycloartanol and cyclosadol were shown to be synthesized by two different SMT enzymes.

UR - http://www.scopus.com/inward/record.url?scp=0030590461&partnerID=8YFLogxK

U2 - 10.1016/0040-4039(96)01534-1

DO - 10.1016/0040-4039(96)01534-1

M3 - Article

AN - SCOPUS:0030590461

SN - 0040-4039

VL - 37

SP - 6823

EP - 6826

JO - Tetrahedron Letters

JF - Tetrahedron Letters

IS - 38

ER -

Phytosterol biosynthesis: Isotope effects associated with biomethylation formation to 24-alkene sterol isomers

Abstract

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