TY - JOUR
T1 - Phosphate starvation induces a determinate developmental program in the roots of Arabidopsis thaliana
AU - Sánchez-Calderón, Lenin
AU - López-Bucio, José
AU - Chacón-López, Alejandra
AU - Cruz-Ramírez, Alfredo
AU - Nieto-Jacobo, Fernanda
AU - Dubrovsky, Joseph G.
AU - Herrera-Estrella, Luis
N1 - Funding Information:
We thank Liana Contreras Burciaga and Selene Napsucialy Men-divil for excellent technical help. We also thank June Simpson for critically reviewing our manuscript, Peter Doerner, Ben Scheres, Tom Guilfoyle and Kaschandra Ragothama for kindly providing us with seeds of transgenic Arabidopsis lines, and CIBNOR (La Paz, B.C.S., Mexico) for permission to use a Leica microtome. L.R.H.E. was supported in part by the Consejo Nacional de Ciencia y Tecnologia, Méx-ico (Grant no. 31628-B), the European Commission (Grant no. ICA-4-CT2000–30017) and by the Howard Hughes Medical Institute (grant no. Nbr55003677), and J.G.D. was supported by DGAPA-PAPIIT Project IN 210202 (UNAM).
PY - 2005/1
Y1 - 2005/1
N2 - When growing under limiting phosphate (P) conditions, Arabidopsis thaliana plants show dramatic changes in root architecture, including a reduction in primary root length, increased formation of lateral roots and greater formation of root hairs. Here we report that primary root growth inhibition by low P is caused by a shift from an indeterminate to a determinate developmental program. In the primary root, the low P-induced determinate growth program initiates with a reduction of cell elongation followed by the progressive loss of meristematic cells. At later stages, cell proliferation ceases and cell differentiation takes place at the former cell elongation and meristematic regions of the primary root. In low P, not only the primary but also almost all mature lateral roots enter the determinate developmental program. Kinetic studies of expression of the cell cycle marker CycB1;1:uidA and the quiescent center (QC) identity marker QC46:GUS showed that in low P conditions, reduction in proliferation in the primary root was preceded by alterations in the QC. These results suggest that in Arabidopsis, P limitation can induce a determinate root developmental program that plays an important role in altering root system architecture and that the QC could act as a sensor of environmental signals. JSPP
AB - When growing under limiting phosphate (P) conditions, Arabidopsis thaliana plants show dramatic changes in root architecture, including a reduction in primary root length, increased formation of lateral roots and greater formation of root hairs. Here we report that primary root growth inhibition by low P is caused by a shift from an indeterminate to a determinate developmental program. In the primary root, the low P-induced determinate growth program initiates with a reduction of cell elongation followed by the progressive loss of meristematic cells. At later stages, cell proliferation ceases and cell differentiation takes place at the former cell elongation and meristematic regions of the primary root. In low P, not only the primary but also almost all mature lateral roots enter the determinate developmental program. Kinetic studies of expression of the cell cycle marker CycB1;1:uidA and the quiescent center (QC) identity marker QC46:GUS showed that in low P conditions, reduction in proliferation in the primary root was preceded by alterations in the QC. These results suggest that in Arabidopsis, P limitation can induce a determinate root developmental program that plays an important role in altering root system architecture and that the QC could act as a sensor of environmental signals. JSPP
KW - Arabidopsis
KW - Cell division
KW - Determinate growth
KW - Phosphate availability
KW - Root meristem
UR - http://www.scopus.com/inward/record.url?scp=14644445177&partnerID=8YFLogxK
U2 - 10.1093/pcp/pci011
DO - 10.1093/pcp/pci011
M3 - Article
C2 - 15659445
AN - SCOPUS:14644445177
SN - 0032-0781
VL - 46
SP - 174
EP - 184
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 1
ER -