Phosphate Starvation-Dependent Iron Mobilization Induces CLE14 Expression to Trigger Root Meristem Differentiation through CLV2/PEPR2 Signaling

Dolores Gutiérrez-Alanís; Lenin Yong-Villalobos; Pedro Jiménez-Sandoval; Fulgencio Alatorre-Cobos; Araceli Oropeza-Aburto; Javier Mora-Macías; Federico Sánchez-Rodríguez; Alfredo Cruz-Ramírez; Luis Herrera-Estrella

doi:10.1016/j.devcel.2017.05.009

Phosphate Starvation-Dependent Iron Mobilization Induces CLE14 Expression to Trigger Root Meristem Differentiation through CLV2/PEPR2 Signaling

Dolores Gutiérrez-Alanís, Lenin Yong-Villalobos, Pedro Jiménez-Sandoval, Fulgencio Alatorre-Cobos, Araceli Oropeza-Aburto, Javier Mora-Macías, Federico Sánchez-Rodríguez, Alfredo Cruz-Ramírez, Luis Herrera-Estrella

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

Low inorganic phosphate (Pi) availability causes terminal differentiation of the root apical meristem (RAM), a phenomenon known as root meristem exhaustion or determined growth. Here, we report that the CLE14 peptide acts as a key player in this process. Low Pi stress induces iron mobilization in the RAM through the action of LPR1/LPR2, causing expression of CLE14 in the proximal meristem region. CLV2 and PEPR2 receptors perceive CLE14 and trigger RAM differentiation, with concomitant downregulation of SHR/SCR and PIN/AUXIN pathway. Our results reveal multiple steps of the molecular mechanism of one of the most physiologically important root nutrient responses.

Original language	English
Pages (from-to)	555-570.e3
Journal	Developmental Cell
Volume	41
Issue number	5
DOIs	https://doi.org/10.1016/j.devcel.2017.05.009
State	Published - Jun 5 2017

Keywords

CLE peptides
cell differentiation
phosphate starvation
root development
root stem cells

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10.1016/j.devcel.2017.05.009

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Gutiérrez-Alanís, D., Yong-Villalobos, L., Jiménez-Sandoval, P., Alatorre-Cobos, F., Oropeza-Aburto, A., Mora-Macías, J., Sánchez-Rodríguez, F., Cruz-Ramírez, A., & Herrera-Estrella, L. (2017). Phosphate Starvation-Dependent Iron Mobilization Induces CLE14 Expression to Trigger Root Meristem Differentiation through CLV2/PEPR2 Signaling. Developmental Cell, 41(5), 555-570.e3. https://doi.org/10.1016/j.devcel.2017.05.009

@article{10bdab4659f047e792ea026522fafa5a,

title = "Phosphate Starvation-Dependent Iron Mobilization Induces CLE14 Expression to Trigger Root Meristem Differentiation through CLV2/PEPR2 Signaling",

abstract = "Low inorganic phosphate (Pi) availability causes terminal differentiation of the root apical meristem (RAM), a phenomenon known as root meristem exhaustion or determined growth. Here, we report that the CLE14 peptide acts as a key player in this process. Low Pi stress induces iron mobilization in the RAM through the action of LPR1/LPR2, causing expression of CLE14 in the proximal meristem region. CLV2 and PEPR2 receptors perceive CLE14 and trigger RAM differentiation, with concomitant downregulation of SHR/SCR and PIN/AUXIN pathway. Our results reveal multiple steps of the molecular mechanism of one of the most physiologically important root nutrient responses.",

keywords = "CLE peptides, cell differentiation, phosphate starvation, root development, root stem cells",

author = "Dolores Guti{\'e}rrez-Alan{\'i}s and Lenin Yong-Villalobos and Pedro Jim{\'e}nez-Sandoval and Fulgencio Alatorre-Cobos and Araceli Oropeza-Aburto and Javier Mora-Mac{\'i}as and Federico S{\'a}nchez-Rodr{\'i}guez and Alfredo Cruz-Ram{\'i}rez and Luis Herrera-Estrella",

note = "Funding Information: The authors wish to thank Dr. Ben Scheres and Dr. Frans Tax for providing published transgenic lines (pSHR::SHR∼GFP, pSCR::SCR∼GFP, pPIN1::PIN1∼GFP, pPIN2::PIN2∼GFP, pPIN3::PIN3∼GFP, and pPIN7::PIN7∼GFP; sol2-1, pepr2-1, and clv2-3); Dr. David King for a sample of synthetic CLE14 peptide; Dr. Lopez-Bucio for providing paraquat reagent and the lpr1lpr2 double mutant; Dr. Jia Li for providing the pPEPR2::GUS transgenic line; Dr. Marcos Castellanos-Uribe for his help in obtaining pCLE14::CLE14∼GFP; Dr. Stewart Gillmor for language editing of the manuscript; and M.J. Ortega for technical support. D.A. was supported by a CONACYT (Mexico) PhD fellowship. This work was supported in part by grants from the Howard Hughes Medical Institute (grant 55005946 to L.H.-E.) and CONACyT. Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2017",

month = jun,

day = "5",

doi = "10.1016/j.devcel.2017.05.009",

language = "English",

volume = "41",

pages = "555--570.e3",

journal = "Developmental Cell",

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Gutiérrez-Alanís, D, Yong-Villalobos, L, Jiménez-Sandoval, P, Alatorre-Cobos, F, Oropeza-Aburto, A, Mora-Macías, J, Sánchez-Rodríguez, F, Cruz-Ramírez, A & Herrera-Estrella, L 2017, 'Phosphate Starvation-Dependent Iron Mobilization Induces CLE14 Expression to Trigger Root Meristem Differentiation through CLV2/PEPR2 Signaling', Developmental Cell, vol. 41, no. 5, pp. 555-570.e3. https://doi.org/10.1016/j.devcel.2017.05.009

TY - JOUR

T1 - Phosphate Starvation-Dependent Iron Mobilization Induces CLE14 Expression to Trigger Root Meristem Differentiation through CLV2/PEPR2 Signaling

AU - Gutiérrez-Alanís, Dolores

AU - Yong-Villalobos, Lenin

AU - Jiménez-Sandoval, Pedro

AU - Alatorre-Cobos, Fulgencio

AU - Oropeza-Aburto, Araceli

AU - Mora-Macías, Javier

AU - Sánchez-Rodríguez, Federico

AU - Cruz-Ramírez, Alfredo

AU - Herrera-Estrella, Luis

N1 - Funding Information: The authors wish to thank Dr. Ben Scheres and Dr. Frans Tax for providing published transgenic lines (pSHR::SHR∼GFP, pSCR::SCR∼GFP, pPIN1::PIN1∼GFP, pPIN2::PIN2∼GFP, pPIN3::PIN3∼GFP, and pPIN7::PIN7∼GFP; sol2-1, pepr2-1, and clv2-3); Dr. David King for a sample of synthetic CLE14 peptide; Dr. Lopez-Bucio for providing paraquat reagent and the lpr1lpr2 double mutant; Dr. Jia Li for providing the pPEPR2::GUS transgenic line; Dr. Marcos Castellanos-Uribe for his help in obtaining pCLE14::CLE14∼GFP; Dr. Stewart Gillmor for language editing of the manuscript; and M.J. Ortega for technical support. D.A. was supported by a CONACYT (Mexico) PhD fellowship. This work was supported in part by grants from the Howard Hughes Medical Institute (grant 55005946 to L.H.-E.) and CONACyT. Publisher Copyright: © 2017 Elsevier Inc.

PY - 2017/6/5

Y1 - 2017/6/5

N2 - Low inorganic phosphate (Pi) availability causes terminal differentiation of the root apical meristem (RAM), a phenomenon known as root meristem exhaustion or determined growth. Here, we report that the CLE14 peptide acts as a key player in this process. Low Pi stress induces iron mobilization in the RAM through the action of LPR1/LPR2, causing expression of CLE14 in the proximal meristem region. CLV2 and PEPR2 receptors perceive CLE14 and trigger RAM differentiation, with concomitant downregulation of SHR/SCR and PIN/AUXIN pathway. Our results reveal multiple steps of the molecular mechanism of one of the most physiologically important root nutrient responses.

AB - Low inorganic phosphate (Pi) availability causes terminal differentiation of the root apical meristem (RAM), a phenomenon known as root meristem exhaustion or determined growth. Here, we report that the CLE14 peptide acts as a key player in this process. Low Pi stress induces iron mobilization in the RAM through the action of LPR1/LPR2, causing expression of CLE14 in the proximal meristem region. CLV2 and PEPR2 receptors perceive CLE14 and trigger RAM differentiation, with concomitant downregulation of SHR/SCR and PIN/AUXIN pathway. Our results reveal multiple steps of the molecular mechanism of one of the most physiologically important root nutrient responses.

KW - CLE peptides

KW - cell differentiation

KW - phosphate starvation

KW - root development

KW - root stem cells

UR - http://www.scopus.com/inward/record.url?scp=85020162514&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2017.05.009

DO - 10.1016/j.devcel.2017.05.009

M3 - Article

C2 - 28586647

AN - SCOPUS:85020162514

SN - 1534-5807

VL - 41

SP - 555-570.e3

JO - Developmental Cell

JF - Developmental Cell

IS - 5

ER -

Phosphate Starvation-Dependent Iron Mobilization Induces CLE14 Expression to Trigger Root Meristem Differentiation through CLV2/PEPR2 Signaling

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Keywords

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