@article{9dee4888fdea48209ccc8dcbc4dc405c,
title = "Peanut seed coat acts as a physical and biochemical barrier against aspergillus flavus infection",
abstract = "Aflatoxin contamination is a global menace that adversely affects food crops and human health. Peanut seed coat is the outer layer protecting the cotyledon both at pre‐ and post‐harvest stages from biotic and abiotic stresses. The aim of the present study is to investigate the role of seed coat against A. flavus infection. In‐vitro seed colonization (IVSC) with and without seed coat showed that the seed coat acts as a physical barrier, and the developmental series of peanut seed coat showed the formation of a robust multilayered protective seed coat. Radial growth bioassay revealed that both insoluble and soluble seed coat extracts from 55‐437 line (resistant) showed higher A. flavus inhibition compared to TMV‐2 line (susceptible). Further analysis of seed coat biochemicals showed that hydroxycinnamic and hydroxybenzoic acid derivatives are the predominant phenolic compounds, and addition of these compounds to the media inhibited A. flavus growth. Gene expression analysis showed that genes involved in lignin monomer, proanthocyanidin, and flavonoid biosynthesis are highly abundant in 55‐437 compared to TMV‐2 seed coats. Overall, the present study showed that the seed coat acts as a physical and biochemical barrier against A. flavus infection and its potential use in mitigating the aflatoxin contamination.",
keywords = "Aflatoxin, Aspergillus flavus, Peanut, Seed coat",
author = "Leslie Commey and Tengey, {Theophilus K.} and Cobos, {Christopher J.} and Lavanya Dampanaboina and Dhillon, {Kamalpreet K.} and Pandey, {Manish K.} and Sudini, {Hari Kishan} and Hamidou Falalou and Varshney, {Rajeev K.} and Burow, {Mark D.} and Venugopal Mendu",
note = "Funding Information: Funding: This research is funded by the United States Agency for International Development (USAID) through Cooperative Agreement No. 7200AA 18CA00003 to the University of Georgia as management entity for U.S. Feed the Future Innovation Lab for Peanut (2018‐2023). The contents are the responsibility of the authors and do not necessarily reflect the views of USAID or the United States Government. This research was also supported by CGIAR‐US University linkage grant pro‐ gram (AID‐BFS‐G‐11‐00002‐11). Funding Information: This research is funded by the United States Agency for International Development (USAID) through Cooperative Agreement No. 7200AA 18CA00003 to the University of Georgia as management entity for U.S. Feed the Future Innovation Lab for Peanut (2018?2023). The contents are the responsibility of the authors and do not necessarily reflect the views of USAID or the United States Government. This research was also supported by CGIAR?US University linkage grant program (AID?BFS?G?11?00002?11). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = dec,
doi = "10.3390/jof7121000",
language = "English",
volume = "7",
journal = "Journal of Fungi",
issn = "2309-608X",
number = "12",
}