Abstract
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.
Original language | English |
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Pages (from-to) | 23-38 |
Number of pages | 16 |
Journal | Methods in Molecular Biology |
Volume | 1241 |
DOIs | |
State | Published - 2015 |
Keywords
- Copy number
- Mitochondrial DNA
- Mitochondrial disease
- Mitochondrial toxicity
- MtDNA
- MtDNA depletion
- QPCR