PCR based determination of mitochondrial DNA copy number in multiple species

John P. Rooney; Ian T. Ryde; Laurie H. Sanders; Evan V. Howlett; Meryl D. Colton; Kaylyn E. Germ; Greg D. Mayer; J. Timothy Greenamyre; Joel N. Meyer

doi:10.1007/978-1-4939-1875-1_3

PCR based determination of mitochondrial DNA copy number in multiple species

John P. Rooney, Ian T. Ryde, Laurie H. Sanders, Evan V. Howlett, Meryl D. Colton, Kaylyn E. Germ, Greg D. Mayer, J. Timothy Greenamyre, Joel N. Meyer

Environmental Toxicology

Research output: Contribution to journal › Article › peer-review

157 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.

Original language	English
Pages (from-to)	23-38
Number of pages	16
Journal	Methods in Molecular Biology
Volume	1241
DOIs	https://doi.org/10.1007/978-1-4939-1875-1_3
State	Published - 2015

Keywords

Copy number
Mitochondrial DNA
Mitochondrial disease
Mitochondrial toxicity
MtDNA
MtDNA depletion
QPCR

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10.1007/978-1-4939-1875-1_3

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title = "PCR based determination of mitochondrial DNA copy number in multiple species",

abstract = "Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.",

keywords = "Copy number, Mitochondrial DNA, Mitochondrial disease, Mitochondrial toxicity, MtDNA, MtDNA depletion, QPCR",

author = "Rooney, {John P.} and Ryde, {Ian T.} and Sanders, {Laurie H.} and Howlett, {Evan V.} and Colton, {Meryl D.} and Germ, {Kaylyn E.} and Mayer, {Greg D.} and {Timothy Greenamyre}, J. and Meyer, {Joel N.}",

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AU - Rooney, John P.

AU - Ryde, Ian T.

AU - Sanders, Laurie H.

AU - Howlett, Evan V.

AU - Colton, Meryl D.

AU - Germ, Kaylyn E.

AU - Mayer, Greg D.

AU - Timothy Greenamyre, J.

AU - Meyer, Joel N.

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PY - 2015

Y1 - 2015

N2 - Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.

AB - Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.

KW - Copy number

KW - Mitochondrial DNA

KW - Mitochondrial disease

KW - Mitochondrial toxicity

KW - MtDNA

KW - MtDNA depletion

KW - QPCR

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -

PCR based determination of mitochondrial DNA copy number in multiple species

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Keywords

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