PCR based determination of mitochondrial DNA copy number in multiple species

John P. Rooney, Ian T. Ryde, Laurie H. Sanders, Evan V. Howlett, Meryl D. Colton, Kaylyn E. Germ, Greg D. Mayer, J. Timothy Greenamyre, Joel N. Meyer

Research output: Contribution to journalArticlepeer-review

157 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.

Original languageEnglish
Pages (from-to)23-38
Number of pages16
JournalMethods in Molecular Biology
Volume1241
DOIs
StatePublished - 2015

Keywords

  • Copy number
  • Mitochondrial DNA
  • Mitochondrial disease
  • Mitochondrial toxicity
  • MtDNA
  • MtDNA depletion
  • QPCR

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