PCR based determination of mitochondrial DNA copy number in multiple species

John P. Rooney; Ian T. Ryde; Laurie H. Sanders; Evan H. Howlett; Meryl D. Colton; Kaylyn E. Germ; Greg D. Mayer; J. Timothy Greenamyre; Joel N. Meyer

doi:10.1007/978-1-4939-1875-1-3

PCR based determination of mitochondrial DNA copy number in multiple species

John P. Rooney, Ian T. Ryde, Laurie H. Sanders, Evan H. Howlett, Meryl D. Colton, Kaylyn E. Germ, Greg D. Mayer, J. Timothy Greenamyre, Joel N. Meyer

Environmental Toxicology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

82 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.

Original language	English
Title of host publication	Mitochondrial Regulation
Subtitle of host publication	Methods and Protocols
Publisher	Springer New York
Pages	23-38
Number of pages	16
ISBN (Electronic)	9781493918751
ISBN (Print)	9781493918744
DOIs	https://doi.org/10.1007/978-1-4939-1875-1-3
State	Published - Oct 11 2014

Keywords

Copy number
Mitochondrial DNA
Mitochondrial disease
Mitochondrial toxicity
QPCR
mtDNA
mtDNA depletion

Access to Document

10.1007/978-1-4939-1875-1-3

Link to publication in Scopus

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abstract = "Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.",

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PCR based determination of mitochondrial DNA copy number in multiple species. / Rooney, John P.; Ryde, Ian T.; Sanders, Laurie H.; Howlett, Evan H.; Colton, Meryl D.; Germ, Kaylyn E.; Mayer, Greg D.; Greenamyre, J. Timothy; Meyer, Joel N.

Mitochondrial Regulation: Methods and Protocols. Springer New York, 2014. p. 23-38.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Sanders, Laurie H.

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AU - Colton, Meryl D.

AU - Germ, Kaylyn E.

AU - Mayer, Greg D.

AU - Greenamyre, J. Timothy

AU - Meyer, Joel N.

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