PCR based determination of mitochondrial DNA copy number in multiple species

John P. Rooney, Ian T. Ryde, Laurie H. Sanders, Evan H. Howlett, Meryl D. Colton, Kaylyn E. Germ, Greg D. Mayer, J. Timothy Greenamyre, Joel N. Meyer

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

82 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.

Original languageEnglish
Title of host publicationMitochondrial Regulation
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages23-38
Number of pages16
ISBN (Electronic)9781493918751
ISBN (Print)9781493918744
DOIs
StatePublished - Oct 11 2014

Keywords

  • Copy number
  • Mitochondrial DNA
  • Mitochondrial disease
  • Mitochondrial toxicity
  • QPCR
  • mtDNA
  • mtDNA depletion

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