Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii

Aimee M. Terauchi, Shu Fen Lu, Mirko Zaffagnini, Shane Tappa, Masakazu Hirasawa, Jitindra N. Tripathy, David B. Knaff, Patrick J. Farmer, Stéphane D. Lemaire, Toshiharu Hase, Sabeeha S. Merchant

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Abstract

Ferredoxin (Fd) is the major iron-containing protein in photosynthetic organisms and is central to reductive metabolism in the chloroplast. The Chlamydomonas reinhardtii genome encodes six plant type [Fe2S2] ferredoxins, products of PETF, FDX2-FDX6. We performed the functional analysis of these ferredoxins by localizing Fd, Fdx2, Fdx3, and Fdx6 to the chloroplast by using isoform-specific antibodies and monitoring the pattern of gene expression by iron and copper nutrition, nitrogen source, and hydrogen peroxide stress. In addition, we also measured the midpoint redox potentials of Fd and Fdx2 and determined the kinetic parameters of their reactions with several ferredoxin-interacting proteins, namely nitrite reductase, Fd:NADP+ oxidoreductase, and Fd:thioredoxin reductase. We found that each of the FDX genes is differently regulated in response to changes in nutrient supply. Moreover, we show that Fdx2 (Em=-321 mV), whose expression is regulated by nitrate, is a more efficient electron donor to nitrite reductase relative to Fd. Overall, the results suggest that each ferredoxin isoform has substrate specificity and that the presence of multiple ferredoxin isoforms allows for the allocation of reducing power to specific metabolic pathways in the chloroplast under various growth conditions.

Original languageEnglish
Pages (from-to)25867-25878
Number of pages12
JournalJournal of Biological Chemistry
Volume284
Issue number38
DOIs
StatePublished - Sep 18 2009

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Terauchi, A. M., Lu, S. F., Zaffagnini, M., Tappa, S., Hirasawa, M., Tripathy, J. N., Knaff, D. B., Farmer, P. J., Lemaire, S. D., Hase, T., & Merchant, S. S. (2009). Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii. Journal of Biological Chemistry, 284(38), 25867-25878. https://doi.org/10.1074/jbc.M109.023622