TY - JOUR
T1 - Parts-per-billion limits of detection via absorbance spectroscopy
T2 - An ultraviolet (254 nm) absorbance detector for liquid chromatography using a light emitting diode (LED)
AU - Thompson, Jonathan E.
N1 - Publisher Copyright:
© Authors.
PY - 2017
Y1 - 2017
N2 - An absorbance detector for high-performance liquid chromatography (HPLC) was developed using a commercially available light emitting diode (LED) at 254 nm. Use of the LED was investigated due to its low output power fluctuation, which should minimize noise and maximize performance. This detector has been characterized and used to perform several separations of relevance to the atmospheric chemistry of airborne particulate matter, specifically, brown carbon (BrC) or humic-like substances (HULIS). The study of atmospheric BrC can be aided by sensitive and general detection schemes, such as absorbance detection. Owing to the exceptional output stability of the LED, the absorbance detector exhibited 3s detection limits of 130 nmol/L (18 ppb) for the chromatography of 4-hydroxybenzoic acid and the detector itself demonstrated noise-equivalent absorption of approx. 180 μAU. The detector was applied to the separation of products resulting from the photo-Fenton reaction of guaiacol. No fewer than 15 individual peaks are noted in the resulting chromatogram. The sensitivity provided is a valuable tool for the chemical analysis of complex samples requiring chromatography.
AB - An absorbance detector for high-performance liquid chromatography (HPLC) was developed using a commercially available light emitting diode (LED) at 254 nm. Use of the LED was investigated due to its low output power fluctuation, which should minimize noise and maximize performance. This detector has been characterized and used to perform several separations of relevance to the atmospheric chemistry of airborne particulate matter, specifically, brown carbon (BrC) or humic-like substances (HULIS). The study of atmospheric BrC can be aided by sensitive and general detection schemes, such as absorbance detection. Owing to the exceptional output stability of the LED, the absorbance detector exhibited 3s detection limits of 130 nmol/L (18 ppb) for the chromatography of 4-hydroxybenzoic acid and the detector itself demonstrated noise-equivalent absorption of approx. 180 μAU. The detector was applied to the separation of products resulting from the photo-Fenton reaction of guaiacol. No fewer than 15 individual peaks are noted in the resulting chromatogram. The sensitivity provided is a valuable tool for the chemical analysis of complex samples requiring chromatography.
KW - Aerosol
KW - Chromatography
KW - LED
KW - UV detection
UR - http://www.scopus.com/inward/record.url?scp=85051024844&partnerID=8YFLogxK
U2 - 10.12973/ejac.2017.00220a
DO - 10.12973/ejac.2017.00220a
M3 - Article
AN - SCOPUS:85051024844
SN - 1306-3057
VL - 12
SP - 901
EP - 911
JO - Eurasian Journal of Analytical Chemistry
JF - Eurasian Journal of Analytical Chemistry
IS - 6
ER -