P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA function

Kristin D. Kasschau, Zhixin Xie, Edwards Allen, Cesar Llave, Elisabeth J. Chapman, Kate A. Krizan, James C. Carrington

Research output: Contribution to journalArticlepeer-review

733 Scopus citations

Abstract

The molecular basis for virus-induced disease in plants has been a long-standing mystery. Infection of Arabidopsis by Turnip mosaic virus (TuMV) induces a number of developmental defects in vegetative and reproductive organs. We found that these defects, many of which resemble those in miRNA-deficient dicer-like1 (dcl1) mutants, were due to the TuMV-encoded RNA-silencing suppressor, P1/HC-Pro. Suppression of RNA silencing is a counterdefensive mechanism that enables systemic infection by TuMV. The suppressor interfered with the activity of miR171 (also known as miRNA39), which directs cleavage of several mRNAs coding for Scarecrow-like transcription factors, by inhibiting miR171-guided nucleolytic function. Out of ten other mRNAs that were validated as miRNA-guided cleavage targets, eight accumulated to elevated levels in the presence of P1/HC-Pro. The basis for TuMV- and other virus-induced disease in plants may be explained, at least partly, by interference with miRNA-controlled developmental pathways that share components with the antiviral RNA-silencing pathway.

Original languageEnglish
Pages (from-to)205-217
Number of pages13
JournalDevelopmental Cell
Volume4
Issue number2
DOIs
StatePublished - Feb 1 2003

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