Overexpression of an Arabidopsis peroxisomal ascorbate peroxidase gene in tobacco increases protection against oxidative stress

Jing Wang, Hong Zhang, Randy D. Allen

Research output: Contribution to journalArticlepeer-review

174 Scopus citations

Abstract

The Arabidopsis gene APX3 that encodes a putative peroxisomal membrane-bound ascorbate peroxidase was expressed in transgenic tobacco plants. APX3-expressing lines had substantial levels of APX3 mRNA and protein. The H2O2 can be converted to more reactive toxic molecules, e.g. ·OH, if it is not quickly removed from plant cells. The expression of APX3 in tobacco could protect leaves from oxidative stress damage caused by aminotriazole which inhibits catalase activity that is found mainly in glyoxysomes and peroxisomes and leads to accumulation of H2O2 in those organelles. However, these plants did not show increased protection from oxidative damage caused by paraquat which leads to the production of reactive oxygen species in chloroplasts. Therefore, protection provided by the expression of APX3 seems to be specific against oxidative stress originated from peroxisomes, not from chloroplasts, which is consistent with the hypothesis that APX3 is a peroxisomal membrane-bound antioxidant enzyme.

Original languageEnglish
Pages (from-to)725-732
Number of pages8
JournalPlant and Cell Physiology
Volume40
Issue number7
DOIs
StatePublished - 1999

Keywords

  • Arabidopsis
  • Ascorbate peroxidase
  • Glyoxysome
  • Oxidative stress
  • Peroxisome

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