Optimization of cultivation conditions of recombinant Escherichia coli strain KPR-42--producer of human gamma-interferon

I. O. Koboziev, U. B. Sorochyns'ka, A. I. Mel'nyk, V. H. Pylypenko, M. I. Spivak

Research output: Contribution to journalArticle

Abstract

Cultivation conditions for recombinant Escherichia coli strain KPR-42 producing human gamma-interferon in liquid nutrient medium were optimized. Glucose content in the medium was optimized by the reason that glucose catabolism products cause the medium acidification. That permitted to prolong fermentation time and increase recombinant protein content in the cells by 23-25%, avoiding expendable enrichment of the medium by nutrient components.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalMikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993)
Volume68
Issue number1
StatePublished - 2006

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