Open-Tubular Capillary Cell Affinity Chromatography: Single and Tandem Blood Cell Separation

Kelong Wang, Molly K Marshall, Griselda Garza, Dimitrios Pappas

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper, an Open-Tubular capillary Cell Affinity Chromatography (OT-CAC) method to enrich and separate target cells is described. Open tubular capillaries coated with anti-CD4, anti-CD14, or anti-CD19 antibodies were used as affinity chromatography columns to separate target blood cells. Cells were eluted using either shear force or bubbles. Bubbles were used to elute the captured cells without diluting the captured cells appreciably, while maintaining viability (the viability of the recovered cells is 85.83±7.34%; cells’ viability was 90.41±3.49% before separation). Several aspects of the OT-CAC method were studied, such as the affinity of one antibody between two different cell lines, the effect of shear force, and the recovery of captured cells. Single- and multi-cell type separations were demonstrated by isolating CD4+ with antiCD4 coated capillary and isolating CD4+ and CD19+ cells with two capillaries in tandem from blood samples. In the one cell type isolation test, an
Original languageEnglish
Pages (from-to)2118-2124
JournalAnalytical Chemistry
StatePublished - 2008

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