Oleic acid in the absence of a PPARγagonist increases adipogenic gene expression in bovine muscle satellite cells

Xiang Z. Li, Yan Yan, Jun F. Zhang, Jian F. Sun, Bin Sun, Chang G. Yan, Seong H. Choi, Bradley J. Johnson, Jong K. Kim, Stephen B. Smith

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We hypothesized that oleic acid (OA) in the absence of a thiazolidinedione (i.e., a synthetic peroxisome proliferator-Activated receptorγ[PPARγ] agonist) would increase adipogenic gene expression in bovine muscle satellite cells (BSC). The BSC were cultured in differentiation medium containing 10 μM ciglitazone (CI), 100 μM OA, or 100 μM OA plus 10 μM CI (CI-OA). Control (CON) BSC were cultured only in differentiation media (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 proteins was confirmed in the BSC by immunofluorescence staining, demonstrating that we had isolated myogenic cells. The OA BSC had lesser paired box 3 (Pax3) and myogenic differentiation 1 expression but greater Pax7 and mygogenin (MYOG) expression (P < 0.05), than the CON BSC. The CI BSC had greater Pax3, Pax7, and MYOG expression than CON BSC (P < 0.05), suggesting that CI would promote BSC myogenesis under pro-myogenic conditions (i.e., when cultured with horse serum). However, both the OA and CI treatments upregulated the expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα) and C/EBPß, sterol regulatory element-binding protein 1, lipoprotein lipase, and glycerol-3-phosphate acyltransferase 3 gene expression, as well as media adiponectin concentration (P < 0.05). The CI, OA, and CI-OA treatments also increased triacylglycerol and lipid droplet accumulation, in spite of upregulation (relative to CON BSC) of adenosine monophosphate-Activated protein kinase alpha-1, perilipin 2 (PLIN2), and PLIN3 in BSC and downregulation of G protein-coupled protein receptor 43, acyl-CoA synthetase long chain family member 3, and stearoyl-CoA desaturase (P < 0.05). These results indicate that OA in the absence of a synthetic PPARγagonist can effectively increase adipogenic gene expression in BSC.

Original languageEnglish
Pages (from-to)4114-4123
Number of pages10
JournalJournal of animal science
Volume97
Issue number10
DOIs
StatePublished - Oct 3 2019

Keywords

  • adipocytes
  • bovine
  • ciglitazone
  • muscle
  • oleic acid
  • satellite cells

Fingerprint Dive into the research topics of 'Oleic acid in the absence of a PPARγagonist increases adipogenic gene expression in bovine muscle satellite cells'. Together they form a unique fingerprint.

  • Cite this

    Li, X. Z., Yan, Y., Zhang, J. F., Sun, J. F., Sun, B., Yan, C. G., Choi, S. H., Johnson, B. J., Kim, J. K., & Smith, S. B. (2019). Oleic acid in the absence of a PPARγagonist increases adipogenic gene expression in bovine muscle satellite cells. Journal of animal science, 97(10), 4114-4123. https://doi.org/10.1093/jas/skz269