Nutritional regulation of somatostatin expression in rainbow trout, Oncorhynchus mykiss

Melissa M. Ehrman, Gregory T. Melroe, Craig A. Moore, Jeffrey D. Kittilson, Mark A. Sheridan

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Previously we characterized three DNAs from the endocrine pancreas (Brockmann body) of rainbow trout (Oncorhynchus mykiss) that encode for distinct preprosomatostatin (PPSS) molecules: one containing somatostatin-14 and its C-terminus (PPSS I) and two containing [Tyr7, Gly10]-somatostatin-14 at their C-termini (PPSS II′ and PPSS II"). In the present study, the regulation of PPSS expression was studied in rainbow trout placed on varying nutritional regimes (fed continuously, fasted, fasted then refed). Fish that were fasted for one week displayed reduced growth compared to their fed counterparts, but no alteration in pancreatic PPSS expression was noted between the two groups. Fish fasted for 4 and 6 weeks also were growth retarded and displayed increased levels of PPSS I mRNA and PPSS II" mRNA compared to fed animals; PPSS II′ mRNA levels were not affected by food deprivation. Refeeding fish for two weeks following 4 weeks of food deprivation restored growth and reduced PPSS I and PPSS II" mRNA expression to levels similar to those displayed by continuously fed fish. Changes in PPSS expression were correlated with increases in plasma SS. These results indicate that nutritional state modulates differential expression of PPSSs.

Original languageEnglish
Pages (from-to)309-314
Number of pages6
JournalFish Physiology and Biochemistry
Volume26
Issue number4
DOIs
StatePublished - 2002

Keywords

  • Fasting
  • Rainbow trout
  • Somatostatin expression

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