TY - JOUR
T1 - Novel short-term effects of adenovirus Ad-36 on hamster lipoproteins
AU - Kapila, M.
AU - Khosla, P.
AU - Dhurandhar, N. V.
PY - 2004/12
Y1 - 2004/12
N2 - OBJECTIVE: Human adenovirus Ad-36 induces adiposity and lowers total serum cholesterol in chickens, mice and marmosets and Ad-36 antibodies are associated with human obesity. We examined the early effects of Ad-36 inoculation on plasma cholesterol levels in hamsters fed a hyperlipidemic diet. DESIGN: A total of 32 male Golden Syrian hamsters were divided into two equal weight-matched groups and intranasally inoculated with Ad-36 (INF: infected) or media (CON: control). In each group, the animals were fed either a purified diet (PF, n = 8) 40%en fat ± 194 mg cholesterol/1000 kcal or chow (C, n = 8) ad libitum. Animals were killed 5 weeks postinoculation. RESULTS: Nested PCR assay detected Ad-36 DNA in the lung, liver, visceral adipose tissue and skeletal muscle of the INF group, but not in the CON animals. Ad-36 antibodies were detected in the INF group only. For all animals, total plasma cholesterol (TC) was not significantly affected by Ad-36 treatment (203 ± 92 vs 193 ± 75 mg/dl, P = NS; INF vs CON, respectively). In 5 weeks, Ad-36 infection had no effect on TC concentration in hamsters fed chow (128 ± 39 vs 130 ± 27 mg/dl, INF-C vs CON-C, respectively) or those fed PF (269 ± 70 vs 256 ± 47 mg/dl, INF-P vs CON-P, respectively). However, lipoproteins isolated by density gradient ultracentrifugation showed a greater proportion of LDL cholesterol in INF animals, as compared to CON (28.4 ± 1.6% vs 16.4 ± 1.2%, P = 0.02), regardless of dietary treatment (INF-P vs CON-P: 27.3 ± 2.1 vs 15.7 ± 1.5%, P = 0.07; and INF-C vs CON-C: 29.4 ± 1.2 vs 17.0 ± 1.1%, P = 0.009). This shift appears to be from HDL cholesterol to the LDL fractions. CONCLUSION: These data suggest that in the hamster (a model resembling several aspects of human lipoprotein metabolism), Ad-36 infection may acutely affect the intravascular processing of lipoproteins resulting in a more atherogenic lipoprotein profile.
AB - OBJECTIVE: Human adenovirus Ad-36 induces adiposity and lowers total serum cholesterol in chickens, mice and marmosets and Ad-36 antibodies are associated with human obesity. We examined the early effects of Ad-36 inoculation on plasma cholesterol levels in hamsters fed a hyperlipidemic diet. DESIGN: A total of 32 male Golden Syrian hamsters were divided into two equal weight-matched groups and intranasally inoculated with Ad-36 (INF: infected) or media (CON: control). In each group, the animals were fed either a purified diet (PF, n = 8) 40%en fat ± 194 mg cholesterol/1000 kcal or chow (C, n = 8) ad libitum. Animals were killed 5 weeks postinoculation. RESULTS: Nested PCR assay detected Ad-36 DNA in the lung, liver, visceral adipose tissue and skeletal muscle of the INF group, but not in the CON animals. Ad-36 antibodies were detected in the INF group only. For all animals, total plasma cholesterol (TC) was not significantly affected by Ad-36 treatment (203 ± 92 vs 193 ± 75 mg/dl, P = NS; INF vs CON, respectively). In 5 weeks, Ad-36 infection had no effect on TC concentration in hamsters fed chow (128 ± 39 vs 130 ± 27 mg/dl, INF-C vs CON-C, respectively) or those fed PF (269 ± 70 vs 256 ± 47 mg/dl, INF-P vs CON-P, respectively). However, lipoproteins isolated by density gradient ultracentrifugation showed a greater proportion of LDL cholesterol in INF animals, as compared to CON (28.4 ± 1.6% vs 16.4 ± 1.2%, P = 0.02), regardless of dietary treatment (INF-P vs CON-P: 27.3 ± 2.1 vs 15.7 ± 1.5%, P = 0.07; and INF-C vs CON-C: 29.4 ± 1.2 vs 17.0 ± 1.1%, P = 0.009). This shift appears to be from HDL cholesterol to the LDL fractions. CONCLUSION: These data suggest that in the hamster (a model resembling several aspects of human lipoprotein metabolism), Ad-36 infection may acutely affect the intravascular processing of lipoproteins resulting in a more atherogenic lipoprotein profile.
KW - Cholesterol
KW - HDL
KW - Hamster
KW - Hypocholesterolemia
KW - Hypolipidemia
KW - LDL
KW - Lipid
UR - http://www.scopus.com/inward/record.url?scp=9444265902&partnerID=8YFLogxK
U2 - 10.1038/sj.ijo.0802710
DO - 10.1038/sj.ijo.0802710
M3 - Article
C2 - 15467779
AN - SCOPUS:9444265902
SN - 0307-0565
VL - 28
SP - 1521
EP - 1527
JO - International Journal of Obesity
JF - International Journal of Obesity
IS - 12
ER -