TY - JOUR
T1 - Novel fluorescence resonance energy transfer optical sensors for vitamin B12 detection using thermally reduced carbon dots
AU - Wang, Jilong
AU - Wei, Junhua
AU - Su, Siheng
AU - Qiu, Jingjing
N1 - Publisher Copyright:
© The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2015.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - In this paper, a novel thermally-reduced carbon dot (t-CD) based fluorescence resonance energy transfer (FRET) sensor for the determination of vitamin B12 (VB12) in aqueous solutions is reported. Carbon dots (CDs) have attracted great attention due to their excellent tunable optical properties, low cost, easy fabrication and low toxicity, which make them ideal candidates for optical sensors. Through esterification reactions, blue luminescent t-CDs were prepared by the carbonization of citric acid and thermally reduced by a thermogravimetric analyzer. After thermal reduction, the quantum yield of the t-CDs demonstrated a 5-fold increase, which makes t-CDs excellent donors in the FRET process. The t-CDs were used to detect VB12 with concentrations ranging from 1 to 12 μg ml-1 and their limit of detection (LOD) was as low as 0.1 μg ml-1. The as-synthesized t-CD based optical probing technique is demonstrated to be simple, cost-effective, sensitive and selective for the detection of biologically significant VB12.
AB - In this paper, a novel thermally-reduced carbon dot (t-CD) based fluorescence resonance energy transfer (FRET) sensor for the determination of vitamin B12 (VB12) in aqueous solutions is reported. Carbon dots (CDs) have attracted great attention due to their excellent tunable optical properties, low cost, easy fabrication and low toxicity, which make them ideal candidates for optical sensors. Through esterification reactions, blue luminescent t-CDs were prepared by the carbonization of citric acid and thermally reduced by a thermogravimetric analyzer. After thermal reduction, the quantum yield of the t-CDs demonstrated a 5-fold increase, which makes t-CDs excellent donors in the FRET process. The t-CDs were used to detect VB12 with concentrations ranging from 1 to 12 μg ml-1 and their limit of detection (LOD) was as low as 0.1 μg ml-1. The as-synthesized t-CD based optical probing technique is demonstrated to be simple, cost-effective, sensitive and selective for the detection of biologically significant VB12.
UR - http://www.scopus.com/inward/record.url?scp=84919332603&partnerID=8YFLogxK
U2 - 10.1039/c4nj00538d
DO - 10.1039/c4nj00538d
M3 - Article
AN - SCOPUS:84919332603
VL - 39
SP - 501
EP - 507
JO - New Journal of Chemistry
JF - New Journal of Chemistry
SN - 1144-0546
IS - 1
ER -