Nitrous oxide emission measurement with acetylene inhibition method in paddy fields under flood conditions

Toshiaki Iida, Sanjit Kumar Deb, Ram Gopal Kharbuja

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8 Scopus citations


Nitrous oxide (N2O) emission from flooded rice paddy fields was continuously measured by the closed chamber method at an experimental plot in Thailand for a whole cultivation period. To characterize the N2O emission with regard to the denitrification N loss, the C2H 2 inhibition method was applied. Flood water on the soil greatly suppressed the N2O emission. The N2O emission was mitigated considerably by even a thin film of the flood water. The overall average N2O emissions under flood conditions for one crop season (83 days) at the control site and the C2H2 treated site were 10.3 and 11.8 μg N m-2 h-1, respectively. The N 2O emission from the C2H2 treated site was consistently higher than that from the control site and the N2O emission from both sites followed the same diurnal and seasonal variation pattern, indicating the effect of denitrification inhibition by the supplied C2H2. The N2O emission enhanced along with temperature increase when NO3-N concentration in the soil water was above 0.4 mg N l-1 and soil temperature was above 24°C, suggesting specific temperature influence over the emission. The increase in NO3-N concentration and temperature in the soil affected only the N2O emission while the difference in the emission at the C 2H2 treated site and the control site was not so much affected. It was suggested that most of the actively produced N2O under higher NO3-N concentration and temperature would likely to quickly emit to the atmosphere rather than to undergo further reduction to N2.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalPaddy and Water Environment
Issue number2
StatePublished - Jun 2007


  • Acetylene inhibition method
  • Flooded period
  • Flooded soil
  • Nitrous oxide emission
  • Paddy field
  • Rice cultivation


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