Next Generation Sequencing (NGS) Application in Multiparameter Gene Expression Analysis

Degeng Wang, Andrey Karamyshev

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Next generation sequencing (NGS) is routinely used in gene expression analyses. In particular, RNA-seq has been the method of choice for highly sensitive genome-wide quantification of RNA expression. The method can be used in a wide variety of model systems, including studies to gain insight into underlying mechanisms of toxicologic processes and disease development induced by environmental toxicants. RNA-seq has also been coupled to many other molecular biology protocols to monitor specific aspects of the gene expression process. Here, we describe two such coupling—(a) global run-on sequencing (GRO-seq) that coupled it to the nuclear run-on (NRO), and (b) polysome profiling that coupled it to sucrose-gradient-based polysome isolation. Simultaneous RNA-seq, GRO-seq, and polysome profiling analyses enabled genome-wide analysis of the mode of stability control of individual RNA molecules.
Original languageEnglish
Title of host publicationNext Generation Sequencing (NGS) Application in Multiparameter Gene Expression Analysis
PublisherSpringer Nature
Volume2102
StatePublished - Jan 28 2020

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