@inbook{023afa8d67774e5fa6cd1b7dc4d596d1,
title = "Next generation sequencing (NGS) application in multiparameter gene expression analysis",
abstract = "Next generation sequencing (NGS) is routinely used in gene expression analyses. In particular, RNA-seq has been the method of choice for highly sensitive genome-wide quantification of RNA expression. The method can be used in a wide variety of model systems, including studies to gain insight into underlying mechanisms of toxicologic processes and disease development induced by environmental toxicants. RNA-seq has also been coupled to many other molecular biology protocols to monitor specific aspects of the gene expression process. Here, we describe two such coupling—(a) global run-on sequencing (GRO-seq) that coupled it to the nuclear run-on (NRO), and (b) polysome profiling that coupled it to sucrose-gradient-based polysome isolation. Simultaneous RNA-seq, GRO-seq, and polysome profiling analyses enabled genome-wide analysis of the mode of stability control of individual RNA molecules.",
keywords = "GRO-seq, Gene expression regulation, Next generation sequencing, Nuclear run-on, Polysome profiling, Polysomes, RNA-seq, Ribosomes, Sucrose gradient, Translation",
author = "Degeng Wang and Karamychev, {Andrey L.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0223-2_2",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "17--34",
booktitle = "Methods in Molecular Biology",
}