New hyphenated methodologies in high-sensitivity glycoprotein analysis

Milos V. Novotny, Yehia Mechref

Research output: Contribution to journalReview article

92 Scopus citations

Abstract

High-sensitivity glycoprotein analyses are of particular interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of new hyphenated methodologies in high-sensitivity glycoprotein analysis is highlighted in this thematic review. These methodologies include, in particular, capillary LC/MALDI/TOF/TOF MS in conjunction with online permethylation platform, and silica-based lectin microcolumns interfaced to MS. The potential of these methodologies in glycomic and glycoproteomic analysis is demonstrated for model glycoproteins as well as total glycomes and glycoproteomes derived from biological samples. Additionally, the applications of CE-MS, CEC, and nanoLC with graphitized carbon in the areas of glycomics and glycoproteomics are described.

Original languageEnglish
Pages (from-to)1956-1968
Number of pages13
JournalJournal of Separation Science
Volume28
Issue number15
DOIs
StatePublished - Oct 2005

Keywords

  • Glycomics
  • Glycoproteins
  • LC/MALDI/TOF/TOF/ MS
  • LC/MS
  • Lectin
  • Oligosaccharides
  • Online permenthylation
  • Review
  • Tandem mass spectrometry

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