New hyphenated methodologies in high-sensitivity glycoprotein analysis

Milos V. Novotny, Yehia Mechref

Research output: Contribution to journalReview articlepeer-review

95 Scopus citations


High-sensitivity glycoprotein analyses are of particular interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of new hyphenated methodologies in high-sensitivity glycoprotein analysis is highlighted in this thematic review. These methodologies include, in particular, capillary LC/MALDI/TOF/TOF MS in conjunction with online permethylation platform, and silica-based lectin microcolumns interfaced to MS. The potential of these methodologies in glycomic and glycoproteomic analysis is demonstrated for model glycoproteins as well as total glycomes and glycoproteomes derived from biological samples. Additionally, the applications of CE-MS, CEC, and nanoLC with graphitized carbon in the areas of glycomics and glycoproteomics are described.

Original languageEnglish
Pages (from-to)1956-1968
Number of pages13
JournalJournal of Separation Science
Issue number15
StatePublished - Oct 2005


  • Glycomics
  • Glycoproteins
  • LC/MS
  • Lectin
  • Oligosaccharides
  • Online permenthylation
  • Review
  • Tandem mass spectrometry


Dive into the research topics of 'New hyphenated methodologies in high-sensitivity glycoprotein analysis'. Together they form a unique fingerprint.

Cite this