TY - JOUR
T1 - Negative feedback regulation of Dicer-Like1 in Arabidopsis by microRNA-guided mRNA degradation
AU - Xie, Zhixin
AU - Kasschau, Kristin D.
AU - Carrington, James C.
N1 - Funding Information:
We thank Dr. Xuemei Chen (Rutgers University) for providing hen1-1 seeds. We also thank Lisa Johansen and Cesar Llave for valuable discussions during this study. This work was supported by grants from the National Science Foundation (MCB-0209836), the National Institutes of Health (AI43288), and the U.S. Department of Agriculture (NRI2002-35319-11560).
PY - 2003/4/29
Y1 - 2003/4/29
N2 - Formation of microRNA (miRNA) requires an RNaseIII domain-containing protein, termed DICER-1 in animals and DICER-LIKE1 (DCL1) in plants, to catalyze processing of an RNA precursor with a fold-back structure [1]. Loss-of-function dcl1 mutants of Arabidopsis have low levels of miRNA and exhibit a range of developmental phenotypes in vegetative, reproductive, and embryonic tissues [2]. In this paper, we show that DCL1 mRNA occurs in multiple forms, including truncated molecules that result from aberrant pre-mRNA processing. Both full-length and truncated forms accumulated to relatively low levels in plants containing a functional DCL1 gene. However, in dcl1 mutant plants, dcl1 RNA forms accumulated to levels several-fold higher than those in DCL1 plants. Elevated levels of DCL1 RNAs were also detected in miRNA-defective hen1 mutant plants and in plants expressing a virus-encoded suppressor of RNA silencing (P1/HC-Pro), which inhibits miRNA-guided degradation of target mRNAs. A miRNA (miR162) target sequence was predicted near the middle of DCL1 mRNA, and a DCL1-derived RNA with the properties of a miR162-guided cleavage product was identified and mapped. These results indicate that DCL1 mRNA is subject to negative feedback regulation through the activity of a miRNA.
AB - Formation of microRNA (miRNA) requires an RNaseIII domain-containing protein, termed DICER-1 in animals and DICER-LIKE1 (DCL1) in plants, to catalyze processing of an RNA precursor with a fold-back structure [1]. Loss-of-function dcl1 mutants of Arabidopsis have low levels of miRNA and exhibit a range of developmental phenotypes in vegetative, reproductive, and embryonic tissues [2]. In this paper, we show that DCL1 mRNA occurs in multiple forms, including truncated molecules that result from aberrant pre-mRNA processing. Both full-length and truncated forms accumulated to relatively low levels in plants containing a functional DCL1 gene. However, in dcl1 mutant plants, dcl1 RNA forms accumulated to levels several-fold higher than those in DCL1 plants. Elevated levels of DCL1 RNAs were also detected in miRNA-defective hen1 mutant plants and in plants expressing a virus-encoded suppressor of RNA silencing (P1/HC-Pro), which inhibits miRNA-guided degradation of target mRNAs. A miRNA (miR162) target sequence was predicted near the middle of DCL1 mRNA, and a DCL1-derived RNA with the properties of a miR162-guided cleavage product was identified and mapped. These results indicate that DCL1 mRNA is subject to negative feedback regulation through the activity of a miRNA.
UR - http://www.scopus.com/inward/record.url?scp=0038076109&partnerID=8YFLogxK
U2 - 10.1016/S0960-9822(03)00281-1
DO - 10.1016/S0960-9822(03)00281-1
M3 - Article
C2 - 12725739
AN - SCOPUS:0038076109
SN - 0960-9822
VL - 13
SP - 784
EP - 789
JO - Current Biology
JF - Current Biology
IS - 9
ER -