Abstract
Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, is an effective chemopreventive agent.
The objective of this study was to develop nanoemulsion formulations for the oral delivery of BITC. Optimized oil-in-water BITC
nanoemulsions were prepared by a spontaneous self-nanoemulsification method and a homogenizationsonication method. Both
nanoemulsions entrapped high amounts of BITC (1517 mg/mL), with low polydispersity and good colloidal stability. The BITC
nanoemulsions showed enhanced solubility and dissolution compared to pure BITC. These formulations markedly increased the
apical to basolateral transport of BITC in Caco-2 cell monolayers. The apparent permeability values were 3.6 106 cm/s for pure
BITC and (1.11.3) 105 cm/s for BITC nanoemulsions. The nanoemulsions were easily taken up by human cancer cells A549
and SKOV-3 and inhibited tumor growth in vitro. This work shows for the first time that BITC can be formulated into
nanoemulsions and may show promi
Original language | English |
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Pages (from-to) | 12396-12404 |
Journal | J Agric Food Chem. |
State | Published - Dec 14 2011 |