Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, is an effective chemopreventive agent. The objective of this study was to develop nanoemulsion formulations for the oral delivery of BITC. Optimized oil-in-water BITC nanoemulsions were prepared by a spontaneous self-nanoemulsification method and a homogenizationsonication method. Both nanoemulsions entrapped high amounts of BITC (1517 mg/mL), with low polydispersity and good colloidal stability. The BITC nanoemulsions showed enhanced solubility and dissolution compared to pure BITC. These formulations markedly increased the apical to basolateral transport of BITC in Caco-2 cell monolayers. The apparent permeability values were 3.6 106 cm/s for pure BITC and (1.11.3) 105 cm/s for BITC nanoemulsions. The nanoemulsions were easily taken up by human cancer cells A549 and SKOV-3 and inhibited tumor growth in vitro. This work shows for the first time that BITC can be formulated into nanoemulsions and may show promi
|Journal||J Agric Food Chem.|
|State||Published - Dec 14 2011|
Qhattal, H. S. S., Wang, S., Salihima, T., Srivastava, S. K., & Liu, X. (2011). Nanoemulsions of Cancer Chemopreventive Agent Benzyl Isothiocyanate Display Enhanced Solubility, Dissolution, and Permeability. J Agric Food Chem., 12396-12404.